Francisella tularensis, from the family Francisellaceae, is the aetiological agent of a zoonosis called tularaemia, spread throughout the northern hemisphere. The infectious dose is extremely low (10 CFU/ml) and the infection causes severe diseases or even death if untreated. The transmission to humans is always related to animals, either by a direct contact or by a contact with the environment contaminated by them. Clinical symptoms of the disease can vary depending on the point of entry of the infection. Tularaemia may also occur without any local symptoms or can be manifested by a combination of the symptoms of various typical clinical forms. F. tularensis is a fastidious bacterium and it is rarely diagnosed by blood culture.
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BMC Infect Dis
January 2025
Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, 21702, United States of America.
Background: Point of need diagnostics provide efficient testing capability for remote or austere locations, decreasing the time to answer by minimizing travel or sample transport requirements. Loop-mediated isothermal amplification (LAMP) is an appealing technology for point-of-need diagnostics due to its rapid analysis time and minimal instrumentation requirements.
Methods: Here, we designed and optimized nine LAMP assays that are sensitive and specific to targeted bacterial select agents including Bacillus anthracis, Francisella tularensis, Yersinia pestis, and Brucella spp.
J Vet Res
December 2024
Department of Virology, National Veterinary Research Institute, 24-100 Puławy, Poland.
Introduction: In winter 2021/2022, a wolf population in the primeval Białowieża Forest in Poland was struck by an outbreak of severe mange caused by mixed infestations of and mites. We present an epidemiological analysis of this mange which caused significant morbidity and mortality.
Material And Methods: Ten sites known for wolf activity were monitored by camera trapping.
Front Cell Infect Microbiol
January 2025
Department of Molecular Pathology and Biology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czechia.
Many pathogens have evolved sophisticated strategies to evade autophagy, a crucial cellular defense mechanism that typically targets and degrades invading microorganisms. By subverting or inhibiting autophagy, these pathogens can create a more favorable environment for their replication and survival within the host. For instance, some bacteria secrete factors that block autophagosome formation, while others might escape from autophagosomes before degradation.
View Article and Find Full Text PDFMicroorganisms
December 2024
Targeted Therapy Team, Institute for Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
The COVID-19 and mpox crisis has reminded the world of the potentially catastrophic consequences of biological agents. Aside from the natural risk, biological agents can also be weaponized or used for bioterrorism. Dissemination in a population or among livestock could be used to destabilize a nation by creating a climate of terror, by negatively impacting the economy and undermining institutions.
View Article and Find Full Text PDFPathogens
December 2024
Departamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, Mexico.
Rodents play a significant role in the transmission of zoonotic diseases; anthropization has increased human contact with these animals, vectors of infectious agents. However, the processes driving parasitism of hosts remains poorly understood. , spp.
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