Eleven X-chromosomal STRs from two multiplex PCR approaches (DXS6807, DXS8378, DXS7132, DXS6800, DXS9898, DXS7424, DXS101, DXS7133, HPRTB, DXS8377, and DXS7423), located in four different X-chromosomal linkage groups, were typed in two population samples from Africa, Morocco, and Madagascar. Forensic efficiency parameters such as polymorphism information content and mean exclusion chance were calculated. A deviation from the Hardy–Weinberg equilibrium could not be found. The investigation of four father–daughter and five mother–son meioses (from Morocco) revealed no mutations in any STR analyzed. Our data were compared with European, African-American, and Asian populations from the literature.
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