AI Article Synopsis
- Researchers engineered a tobacco plant by integrating a bacterial merB gene with other components to enhance its ability to remove methylmercury pollutants from the environment.
- The modified tobacco exhibited increased resistance to methylmercury and accumulated more mercury, effectively converting the toxic CH3Hg+ into a less harmful Hg2+ form.
- This genetically modified plant presents a promising phytoremediation solution, capable of safely neutralizing mercury and preventing its release into the atmosphere, which may be more publicly accepted than other methods like phytovolatilization.*
Article Abstract
To develop the potential of plant for phytoremediation of methylmercury pollution, a genetically engineered tobacco plant that coexpresses organomercurial lyase (MerB) with the ppk-specified polyphosphate (polyP) and merT-encoding mercury transporter was constructed by integrating a bacterial merB gene into ppk/merT-transgenic tobacco. A large number of independent transgenic tobaccos was obtained, in some of which the merB gene was stably integrated in the plant genome and substantially translated to the expected MerB enzyme in the transgenic tobacco. The ppk/merT/merB-transgenic tobacco callus showed more resistance to methylmercury (CH3Hg+) and accumulated more mercury from CH3Hg+-containing medium than the ppk/merT-transgenic and wild-type progenitors. These results suggest that the MerB enzyme encoded by merB degraded the incorporated CH3Hg+ to Hg2+, which then accumulated as a less toxic Hg-polyP complex in the tobacco cells. Phytoremediation of CH3Hg+ and Hg2+ in the environment with this engineered ppk/merT/merB-transgenic plant, which prevents the release mercury vapor (Hg0) into the atmosphere in addition to generating potentially recyclable mercury-rich plant residues, is believed to be more acceptable to the public than other competing technologies, including phytovolatilization.
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| http://dx.doi.org/10.1007/s00253-010-2572-9 | DOI Listing |
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