AI Article Synopsis
- The study aimed to explore the frequency of specific TLR2 gene polymorphisms (Arg753Gln and Arg677Trp) in patients with cutaneous leishmaniasis compared to healthy individuals.
- The research used techniques like PCR-RFLP and ARMS-PCR for genotyping, revealing that Arg753Gln was present in 14.3% of CL patients and 10.1% of controls, with no significant difference in frequencies.
- No Arg677Trp polymorphism cases were found in either group, leading to the conclusion that TLR2 mutations occur similarly in both CL patients and healthy individuals.
Article Abstract
The aim of the present study was to investigate the frequency of Arg753Gln and Arg677Trp polymorphisms of TLR2 in patients with cutaneous leishmaniasis (CL) compared to healthy controls. The polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system assay (ARMS-PCR). The results showed that the frequency of Arg753Gln genotype was 14.3% and 10.1% in CL patients and normal controls, respectively. No one in either group was homozygous for the mutation. There was no significant difference in the genotype frequency. In contrast to the results for Arg753Gln polymorphism, we did not detect any case with Arg677Trp polymorphism in either control or patient group. In conclusion the TLR2 mutations are found equally in CL patients and healthy subjects.
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| http://dx.doi.org/10.1016/j.parint.2010.03.008 | DOI Listing |
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