[Influence of gold and silver nanoparticles on ATPase activity of native and rehydrated cells of Escherichia coli].

The expressed changes in the bacteria cells' plasma membrane ATPase activity values cells of Escherichia coli, which are used in the manufacturing of vaccines against cattle colienterotoxemia, have been revealed under the influence of lyophilization/rehydration process. Influence of gold (average size 20, 30, 45 nm) and silver (30 nm) nanoparticles in different concentrations on ATPase activity of native and rehydrated (delyophilized) bacteria cells has been studied. Gold and silver nanoparticles stimulated ATPase activity of native and rehydrated (delyophilizated) cells of the investigated strains. Obtained data show, that application of examined metal nanoparticles of certain sizes and concentrations as protectors and stimulators of main physiological-biochemical properties of production strains' cells under the conditions of lyophilization and long storage stress in technologies of different immunobiological preparations manufacturing is very promising.