A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethylsalicylic acid. This gave good linearity with respect to the concentration of pullulan in the fermentation broth. The content of pullulan determined in this way was less than that determined by a coupled enzyme assay and was comparable to that determined by an HPLC method. The new method was specific for estimation of pullulan, demonstrated high accuracy, and could assay pullulan from up to 3.2 mg/ml.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10529-010-0272-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The food enzyme pullulanase (pullulan 6-α-glucanohydrolase; EC 3.2.1.
View Article and Find Full Text PDFNovel chromosomes and genomes provide new insights into evolution and adaptation of the whole genome duplicated yeast-like fungus TN3-1 isolated from natural honey.
Funct Integr Genomics
June 2023
College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
Aureobasidium melanogenum TN3-1 strain and A. melanogenum P16 strain were isolated from the natural honey and the mangrove ecosystem, respectively. The former can produce much higher pullulan from high concentration of glucose than the latter.
View Article and Find Full Text PDFToxic Ag detection based on Au@Ag core shell nanostructure formation using Tannic acid assisted synthesis of Pullulan stabilized gold nanoparticles.
Sci Rep
February 2023
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Herein, a sensitive colorimetric detection strategy is proposed for Ag detection based on the use of environmentally friendly synthesis of gold nanoparticles (AuNPs), at room temperature, using (tannic acid, TA), as the reductant and pullulan (PUL) as stabilizing agent. The colloidal solution (TA/PUL-AuNPs), at the optimal synthesis conditions, showed maximum absorbance at 529 nm with a berry red color. TEM and FESEM validated that the particles are spherical and monodispersed, while other characterization results elucidated the role of pullulan in the nano-synthesis.
View Article and Find Full Text PDFProduction, Partial Purification, and Characterization of Polygalacturonase from Aureobasidium pullulans P56 under Submerged Fermentation Using Agro-Industrial Wastes.
Curr Microbiol
August 2022
Faculty of Sciences and Letters, Department of Biology, Section of Basic and Industrial Microbiology, Manisa Celal Bayar University, 45030, Manisa, Turkey.
Polygalacturonase (PGase) production by Aureobasidium pullulans P56 under submerged fermentation was investigated using agro-industrial wastes and commercial carbon and nitrogen sources. The maximum PGase concentration was equivalent to 8.6 U/mL that was obtained in presence of citrus pectin at 150 rpm, 30 °C, pH = 5.
View Article and Find Full Text PDFThe food enzyme pullulanase (pullulan 6-α-glucanohydrolase, EC 3.2.1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!