Mps1, a dual-specificity kinase, is required for the proper functioning of the spindle assembly checkpoint and for the maintenance of chromosomal stability. As Mps1 function has been implicated in numerous phases of the cell cycle, the development of a potent, selective small-molecule inhibitor of Mps1 should facilitate dissection of Mps1-related biology. We describe the cellular effects and Mps1 cocrystal structures of new, selective small-molecule inhibitors of Mps1. Consistent with RNAi studies, chemical inhibition of Mps1 leads to defects in Mad1 and Mad2 establishment at unattached kinetochores, decreased Aurora B kinase activity, premature mitotic exit and gross aneuploidy, without any evidence of centrosome duplication defects. However, in U2OS cells having extra centrosomes (an abnormality found in some cancers), Mps1 inhibition increases the frequency of multipolar mitoses. Lastly, Mps1 inhibitor treatment resulted in a decrease in cancer cell viability.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857554 | PMC |
| http://dx.doi.org/10.1038/nchembio.345 | DOI Listing |
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Article Synopsis
- - The study aimed to evaluate the anti-cancer effects of a drug called compound-9, which inhibits a protein known as MPS1/TTK, in gastric cancer cell lines.
- - Through various assays like cell viability, apoptosis, and protein analysis, the researchers found that sensitivity to the inhibitor varied among different genetic profiles of gastric cancer, particularly noting differences between TP53 mutated and wild-type cell lines.
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