AI Article Synopsis
- The 2009 H1N1 pandemic created a need for an effective and straightforward screening method for viral infections to manage public health crises.
- Through bioinformatics, a specific genetic marker was identified to differentiate between swine lineage and human seasonal H1N1 viruses, leading to the development of a TaqMan probe-based testing method.
- The new assay demonstrated high sensitivity (92%) and specificity (100%), effectively detecting low viral loads and streamlining laboratory processes for mass sample analysis.
Article Abstract
Background: The pandemic (H1N1) 2009 has become a threat of public health. To manage rapidly increased infections and disease control, a simple and reliable first-line screening test for viral infection is on urgent demand.
Methods: Through comprehensive bioinformatics analysis, a single nucleotide polymorphism in nucleoprotein gene which differentiates swine lineage virus and human seasonal H1N1 virus was selected as target. A TaqMan probe-based allele discrimination analysis was designed to analyze clinical samples. In total, 93 clinical specimens and 39 viral isolates were used to test the assay efficacy. Traditional viral culture and molecular analysis were used as gold standard.
Results: The testing results showed that the established assay has high sensitivity and specificity (92% and 100%) for pandemic (H1N1) 2009. The assay could detect as low as 5 copies of NP gene of pandemic (H1N1) 2009 or 2 viral particles of human seasonal H1N1.
Conclusion: This assay can be used as a first-line screening and confirmation test for pandemic (H1N1) 2009 virus and human seasonal flu in one-tube reaction. The assay can serve as a convenient method to reduce the burden of PCR manipulation for diagnostic laboratories when large amount of samples need to be analyzed in a short time.
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| http://dx.doi.org/10.1016/j.cca.2010.04.002 | DOI Listing |
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