AI Article Synopsis
- Electric pulses of high voltage and short duration can effectively permeabilize the membranes of organelles in living cells, including endocytotic vesicles.
- In a study with B16 F1 mouse melanoma cells, exposure to 10-20 high-voltage electric pulses led to the release of lucifer yellow from vesicles, indicating successful permeabilization.
- As the number of pulses increased, not only did the endocytotic vesicle membranes become permeabilized, but the plasma membrane also showed signs of permeabilization, hinting at a threshold effect based on pulse count.
Article Abstract
It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were exposed to short, high-voltage electric pulses (from 1 to 20 pulses, 60 ns, 50 kV/cm, repetition frequency 1 kHz). We observed that 10 and 20 such pulses induced permeabilization of membranes of endocytotic vesicles, detected by release of lucifer yellow from the vesicles into the cytosol. Simultaneously, we detected uptake of propidium iodide through plasma membrane in the same cells. With higher number of pulses permeabilization of the membranes of endocytotic vesicles by pulses of given parameters is accompanied by permeabilization of plasma membrane. However, with lower number of pulses only permeabilization of the plasma membrane was detected.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855809 | PMC |
| http://dx.doi.org/10.1007/s11517-010-0599-9 | DOI Listing |
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