A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2884511PMC
http://dx.doi.org/10.1128/JCM.00166-10DOI Listing

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