The choice of a suitable detergent-based membrane mimetic is of crucial importance for high-resolution NMR studies of membrane proteins. The present report describes a new approach of detergent screening. It is based on the comparison of 2D (1)H,(15)N-correlation spectra of a protein in a membrane-bilayer "reference" medium and in "trial" detergent-based environments. The proposed "reference" medium is the lipid-protein nanodisc (LPN) representing nanoscale phospholipid bilayers wrapped around by apolipoprotein A-1. The set of zwitterionic (DPC, DMPC/DHPC), anionic (SDS, LMPG, LPPG), and weakly cationic (LDAO) detergent-based media was screened for their ability to represent the native structure of the isolated voltage-sensing domain (VSD) of the archaeal potassium channel KvAP. The VSD/LPN complexes composed of saturated zwitterionic (DMPC), anionic (DMPG), or a mixture of unsaturated differently charged (POPC/DOPG, 3:1) lipids were used as reference. All assayed detergent media demonstrate similar CD spectra of the domain with a high level (approximately 60%) of overall helicity but different 2D NMR spectra. Using the reference spectrum of the VSD in LPN, we were able to choose the detergent composition in which the membrane-like structure of the VSD is preserved.
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http://dx.doi.org/10.1021/ja9097498 | DOI Listing |
Rev Alerg Mex
December 2024
Escola Superior de Ciências da Saúde ESCS Brasília/DF Brazil, Allergy and Dermatology Outpatient Unit at the Hospital Regional da Asa Norte HRAN - SMHN Q 2.
Objectives: To evaluate the prevalence of methylisothiazolinone sensitivity and associated factors in individuals with suspected allergic contact dermatitis.
Methods: Cross-sectional study based on patch tests, including methylisothiazolinone 0.2%, in 286 participants with suspected allergic contact dermatitis, in Brasília/DF, Brazil, between March/2020 and March/2022.
Trop Anim Health Prod
January 2025
Sub Campus T.T Singh, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan.
The current study was designed to evaluate the effect of particle size (PS) and inclusion level of wheat straw (WS) obtained from genetically improved wheat on the performance and feeding behavior of Sahiwal cows. Twelve multiparous, mid-lactating Sahiwal cows (DIM 135 ± 25, mean ± SD; 12.8 ± 1.
View Article and Find Full Text PDFElife
December 2024
Calico Life Sciences LLC, South San Francisco, United States.
Protein aggregation increases during aging and is a pathological hallmark of many age-related diseases. Protein homeostasis (proteostasis) depends on a core network of factors directly influencing protein production, folding, trafficking, and degradation. Cellular proteostasis also depends on the overall composition of the proteome and numerous environmental variables.
View Article and Find Full Text PDFEnzyme Microb Technol
December 2024
Nanjing Vazyme Biotechnology Company Limited, C1-C2, Hong Feng Science and Technology Park, Nanjing 210046, PR China. Electronic address:
Detergent enzymes have been extensively developed as eco-friendly alternatives to harmful chemicals, with alkaline protease representing a significant portion of detergent enzyme sales. However, the self-cleavage function of alkaline protease impacts its activity and overall application. Therefore, a new rational combinatorial strategy is proposed based on self-molecular docking (Self-ZDOCK) and molecular dynamics (MD) simulations.
View Article and Find Full Text PDFInt Immunopharmacol
January 2025
Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Jiangnan University, Wuxi 214002, China. Electronic address:
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