Objective: To study the changes of glucocorticoid receptor (GR) expression in embryonic rat cortical neurons exposed to transient Mg(2+)-free treatment.
Methods: Six days after rat cortical neuronal cultures, two groups were created based on the medium to which were transiently exposed. The control group was exposed to a physiological solution (PS), and the Mg(2+)-free group was exposed to the same medium as the control group except for the removal of magnesium. The expression of GR mRNA and protein was determined by real-time PCR and immunocytochemistry staining 1, 7 and 12 days after transient Mg(2+)-free treatment.
Results: Compared to the control group, the Mg(2+)-free group displayed the significantly less accumulated optical density (AOD) of GR immunoreactivity 12 days after transient Mg(2+)-free treatment (p<0.05). On the contrary, GR mRNA expression increased significantly 1 and 7 days after transient Mg(2+)-free treatment in the Mg(2+)-free group (p<0.05).
Conclusions: GR expression is modified following Mg-free-induced injury in cultured developing neurons in rats.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
NMDA Receptor-Mediated Ca Flux Attenuated by the NMDA Receptor/TRPM4 Interface Inhibitor Brophenexin.
Pharmacol Res Perspect
December 2024
Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
Transient receptor potential melastatin-4 (TRPM4) forms a complex with N-methyl-D-aspartate receptors (NMDARs) that facilitates NMDAR-mediated neurotoxicity. Here we used pharmacological tools to determine how TRPM4 regulates NMDAR signaling. Brophenexin, a compound that binds to TRPM4 at the NMDAR binding interface, protected hippocampal neurons in culture from NMDA-induced death, consistent with published work.
View Article and Find Full Text PDFRoles of metabotropic glutamate receptor 5 in low [Mg]-induced interictal epileptiform activity in rat hippocampal slices.
Korean J Physiol Pharmacol
September 2024
Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.
Group I metabotropic glutamate receptors (mGluRs) modulate postsynaptic neuronal excitability and epileptogenesis. We investigated roles of group I mGluRs on low extracellular Mg concentration ([Mg])-induced epileptiform activity and neuronal cell death in the CA1 regions of isolated rat hippocampal slices without the entorhinal cortex using extracellular recording and propidium iodide staining. Exposure to Mg-free artificial cerebrospinal fluid can induce interictal epileptiform activity in the CA1 regions of rat hippocampal slices.
View Article and Find Full Text PDFTRPML1 ameliorates seizures-related neuronal injury by regulating autophagy and lysosomal biogenesis via Ca/TFEB signaling pathway.
Biochim Biophys Acta Mol Basis Dis
December 2024
Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou 450052, Henan Province, PR China. Electronic address:
Alterations in autophagy have been observed in epilepsy, although their exact etiopathogenesis remains elusive. Transient Receptor Potential Mucolipin Protein 1 (TRPML1) is an ion channel protein that regulates autophagy and lysosome biogenesis. To explore the role of TRPML1 in seizures-induced neuronal injury and the potential mechanisms involved, an hyperexcitable neuronal model induced by Mg-free solution was used for the study.
View Article and Find Full Text PDFCannabigerolic Acid (CBGA) Inhibits the TRPM7 Ion Channel Through its Kinase Domain.
Function (Oxf)
January 2024
Center for Biomedical Research, The Queen's Medical Center, 1301 Punchbowl St., Honolulu, HI 96813, USA.
Cannabinoids are a major class of compounds produced by the plant Cannabis sativa. Previous work has demonstrated that the main cannabinoids cannabidiol (CBD) and tetrahydrocannabinol (THC) can have some beneficial effects on pain, inflammation, epilepsy, and chemotherapy-induced nausea and vomiting. While CBD and THC represent the two major plant cannabinoids, some hemp varieties with enzymatic deficiencies produce mainly cannabigerolic acid (CBGA).
View Article and Find Full Text PDFExtreme Monovalent Ion Selectivity Via Capacitive Ion Exchange.
Water Res
November 2023
Faculty of Mechanical Engineering, Technion - Israel Institute of Technology, Haifa, Israel; Grand Technion Energy Program, Technion - Israel Institute of Technology, Haifa, Israel; Wolfson Department of Chemical Engineering, Technion - Israel Institute of Technology, Haifa, Israel. Electronic address:
Capacitive deionization (CDI) is an emerging technology applied to brackish water desalination and ion selective separations. A typical CDI cell consists of two microporous carbon electrodes, where ions are stored in charged micropore via electrosorption into electric double layers. For typical feed waters containing mixtures of several cations and anions, some of which are polluting, models are needed to guide cell design for a target separation, given the complex electrosorption dynamics of each species.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!