Effects of environmental stress on stability of tandem repeats in Escherichia coli O157:H7.

Appl Environ Microbiol

Produce Safety and Microbiology Research Unit, USDA Agricultural Research Service, 800 Buchanan St., Albany, CA 94710, USA.

Published: May 2010

Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication but changed after exposure to irradiation, elevated temperatures, and starvation conditions. The pO157 plasmid was frequently lost under these stress conditions. Environmental stresses may increase phylogenetic diversity as measured by MLVA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2869130PMC
http://dx.doi.org/10.1128/AEM.02373-09DOI Listing

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