AI Article Synopsis
- NORE1A is a tumor suppressor protein that interacts with microtubules, playing a role in growth and tumor suppression.
- NORE1A can nucleate microtubules, and this function is influenced by phosphorylation from Aurora A kinase and binding to activated Ras.
- The study suggests a new regulatory network involving NORE1A, Aurora A, Ras, and the microtubule cytoskeleton, highlighting its complex role in cellular processes.
Article Abstract
NORE1A is a Ras-binding protein that belongs to a group of tumor suppressors known as the Ras association domain family. Their growth- and tumor-suppressive function is assumed to be dependent on association with the microtubule cytoskeleton. However, a detailed understanding of this interplay is still missing. Here, we show that NORE1A directly interacts with tubulin and is capable of nucleating microtubules. Strikingly, the ability to stimulate nucleation is regulated in a dual specific way either via phosphorylation of NORE1A within the Ras-binding domain by Aurora A kinase or via binding to activated Ras. We also demonstrate that NORE1A mediates a negative effect of activated Ras on microtubule nucleation. On the basis of our results, we propose a novel regulatory network composed of the tumor suppressor NORE1A, the mitotic kinase Aurora A, the small GTPase Ras, and the microtubule cytoskeleton.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871493 | PMC |
| http://dx.doi.org/10.1074/jbc.M109.081562 | DOI Listing |
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