AI Article Synopsis
- West Nile virus (WNV) is a mosquito-borne RNA virus that can cause illness in humans, with no available antivirals or vaccines to treat it, highlighting the urgent need for effective treatments.
- A high throughput screen was conducted with a library of 13,001 compounds, revealing a primary hit rate of 0.185% for compounds that inhibited virus infection in Vero E6 cells.
- Further evaluation of 26 promising compounds led to the identification of 7 potential small molecule probes targeting WNV, indicating some success in the search for antiviral agents.
Article Abstract
West Nile virus (WNV) is a positive sense, single-stranded RNA virus that can cause illness in humans when transmitted via mosquito vectors. Unfortunately, no antivirals or vaccines are currently available, and therefore efficient and safe antivirals are urgently needed. We developed a high throughput screen to discover small molecule probes that inhibit virus infection of Vero E6 cells. A primary screen of a 13,001 compound library at a 10 microM final concentration was conducted using the 384-well format. Z' values ranged from 0.54-0.83 with a median of 0.74. Average S/B was 17 and S/N for each plate ranged from 10.8 to 23.9. Twenty-six compounds showed a dose response in the HT screen and were further evaluated in a time of addition assay and in a titer reduction assay. Seven compounds showed potential as small molecule probes directed at WNV. The hit rate from the primary screen was 0.185% (24 compounds out of 13,001 compounds) and from the secondary screens was 0.053% (7 out of 13,001 compounds) respectively.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839297 | PMC |
| http://dx.doi.org/10.3390/molecules15031690 | DOI Listing |
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