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A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis.
Nucleic Acids Res
January 2025
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
RNA endonucleases are the rate-limiting initiator of decay for many bacterial mRNAs. However, the positions of cleavage and their sequence determinants remain elusive even for the well-studied Bacillus subtilis. Here we present two complementary approaches-transcriptome-wide mapping of endoribonucleolytic activity and deep mutational scanning of RNA cleavage sites-that reveal distinct rules governing the specificity among B.
View Article and Find Full Text PDFSynthesis, antimicrobial evaluation, ADMET prediction, molecular docking and dynamics studies of pyridine and thiophene moiety-containing chalcones.
R Soc Open Sci
January 2025
Department of Chemistry, Jagannath University, Dhaka 1100, Bangladesh.
In this study, three pyridine- and four thiophene-containing chalcone derivatives were synthesized via Claisen-Schmidt condensation reaction, where five derivatives were new. Different spectral analyses (IR, H NMR, HRMS) clarified the structures and these proposed compounds were screened for antimicrobial activity by the agar disc diffusion technique. Compound was conspicuously active against most of the bacterial and fungal strains.
View Article and Find Full Text PDFdoes not give an advantage to the ectoparasitoid (Say, 1836) when it develops on an infected host.
Bull Entomol Res
January 2025
Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.
The effect of on the viability and antimicrobial activity of the ectoparasitoid was evaluated in laboratory experiments. Two lines of the parasitoid, -infected (W+) and -free (W-), were used. Parasitoid larvae were fed with a host orally infected with a sublethal dose of (Bt) and on the host uninfected with Bt.
View Article and Find Full Text PDFGreen synthesis of silver nanoparticles from the endophytic fungus Panax notoginseng and their antioxidant and antimicrobial activities and effects on cherry tomato preservation.
Int J Food Microbiol
January 2025
College of Animal Science and Technology, Yangtze University, Jingzhou 434023, China. Electronic address:
This study investigated endophytic fungi isolated from the medicinal plant Panax notoginseng. Among these, the endophytic fungus SQ3, identified as Chaetomium globosum, was capable of reducing silver ions to form metallic silver nanoparticles. The green-synthesized silver nanoparticles (AgNPs) presented a distinct surface plasmon resonance peak at 424 nm, with particle sizes between 2.
View Article and Find Full Text PDFPerformance of four thermophilic bacteria for primary sludge hydrolysis: Sludge disintegration and hydrolase activities.
Bioresour Technol
January 2025
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address:
Thermophilic bacteria (TB) pretreatment is an efficient and environmentally friendly way for accelerating sludge hydrolysis. In this study, a complete comparison of the hydrolysis performance of Bacillus sp. AT07-1 (X1), Parageobacillus toebii X2 (X2), Geobacillus kaustophilus X3 (X3) and Parageobacillus toebii R-35642 (X4) was performed.
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