ClC chloride channels and transporters play major roles in cellular excitability, epithelial salt transport, volume, pH, and blood pressure regulation. One family member, ClC-ec1 from Escherichia coli, has been structurally resolved crystallographically and subjected to intensive mutagenetic, crystallographic, and electrophysiological studies. It functions as a Cl(-)/H(+) antiporter, not a Cl(-) channel; however, the molecular mechanism for Cl(-)/H(+) exchange is largely unknown. Using all-atom normal-mode analysis to explore possible mechanisms for this antiport, we propose that Cl(-)/H(+) exchange involves a conformational cycle of alternating exposure of Cl(-) and H(+) binding sites of both ClC pores to the two sides of the membrane. Both pores switch simultaneously from facing outward to facing inward, reminiscent of the standard alternating-access mechanism, which may have direct implications for eukaryotic Cl(-)/H(+) transporters and Cl(-) channels.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2849085 | PMC |
| http://dx.doi.org/10.1016/j.bpj.2009.11.035 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unlabelled: All eukaryotes utilize regulated secretion to release molecular signals packaged in secretory granules for local and remote signaling. An anion shunt conductance was first suggested in secretory granules of bovine chromaffin cells nearly five decades ago. Biochemical identity of this conductance remains undefined.
View Article and Find Full Text PDFMolecular composition and formation mechanism of chlorinated organic compounds in biological waste leachate treated by electrochemical oxidation with a boron-doped diamond anode.
Chemosphere
December 2024
School of Environmental Science and Engineering, Southwest Jiaotong University, Chengdu, 611756, China. Electronic address:
The use of electrochemical oxidation with boron-doped diamond (BDD) as an anode has been demonstrated to be an effective means of removing dissolved organic matter (DOM) from biologically treated waste leachate. However, in the presence of chloride ions, undesired chlorine evolution occurs on the anode; this forms chlorinated DOM, mostly of unknown molecular composition. We investigate the molecular composition and formation mechanism of chlorinated DOM during electrochemical oxidation process of biologically treated leachate DOM.
View Article and Find Full Text PDFMultiscale Responsive Kinetic Modeling: Quantifying Biomolecular Reaction Flux under Varying Electrochemical Conditions.
J Chem Theory Comput
January 2025
Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.
Attaining a complete thermodynamic and kinetic characterization for processes involving multiple interconnected rare-event transitions remains a central challenge in molecular biophysics. This challenge is amplified when the process must be understood under a range of reaction conditions. Herein, we present a novel condition-responsive kinetic modeling framework that can combine the strengths of bottom-up rate quantification from multiscale simulations with top-down solution refinement using both equilibrium and nonequilibrium experimental data.
View Article and Find Full Text PDFDesign and synthesis of a novel isoleucine-derived Schiff base ligand: Structural characterization, molecular docking, and biological activity evaluation.
Int J Health Sci (Qassim)
January 2024
Department of Clinical Laboratory Sciences, College of Applied Medical Science, Jouf University, Aljouf, Saudi Arabia.
Article Synopsis
- Schiff bases are important chemical compounds used in various fields such as catalysis, pigments, and have biological activities like antifungal and antibacterial effects.
- A unique Schiff base ligand was created by combining specific organic compounds, and its complexes were characterized through multiple scientific techniques, demonstrating their structural properties.
- The resulting metal complexes showed enhanced antibacterial and antiproliferative effects, particularly against breast cancer cells, indicating their potential as new therapeutic agents, though more research is necessary to explore their mechanisms.
Construction and Comparative Research of Two MOFs Proton Conducting Materials Containing Nitro Groups.
Chemistry
January 2025
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, 650500, Kunming, People's Republic of China.
In field of electrochemistry, there has been a growing interest in the potential applications of proton-conducting metal-organic frameworks (MOFs). Therefore, how to design and synthesize MOFs with high proton conductivity is considered crucial. In this study, two examples of nitro-containing Cd-based MOFs, MOF-1 {[Cd(TIPE)(NO)Cl(HO)] ⋅ 17HO} and MOF-2 {[Cd(TIPE)(nip)] ⋅ 10HO} (TIPE=1,1,2,2-tetrakis(4-(1H-imidazole-1-yl)phenyl)ethene, Hnip=5-Nitroisophthalic Acid), had been successfully designed and synthesized, and their proton-conducting properties were thoroughly investigated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!