[DNA nanosensor fluorescence imaging microscopy].

Guang Pu Xue Yu Guang Pu Fen Xi

The Key Laboratory of Atomic and Molecular Nanosciences, Ministry of Education, Department of Physics, Tsinghua University, Beijing 100084, China.

Published: January 2010

Quantum dots have many excellent optical properties such as high quantum yield, long fluorescence lifetime, wide excitation spectrum and narrow emission spectrum, tunable emission wavelength and so on, thus have become a newpopular type of fluorescence probes in these years. Quantum-dot-based DNA nanosensor comprising streptavidin-conjugated quantum dots, capture probes with biotin and reporter probes with Cy5 was designed to detect DNA or RNA segments. Capture probes and reporter probes were connected by the target DNA or RNA segments so that quantum dots and Cy5s could be together and FRET (fluorescence resonance energy transfer) could be detected. In the present work, quantum-dot-based DNA nanosensor was combined with ICCD fluorescence microscopy imaging system through the authors' experiments. Using the total internal reflection fluorescence (TIRF), FRET between quantum dots and Cy5s was recorded by ICCD showing that segments of singlestranded target DNA with 30-base length were detected in solution using DNA nanosensor. When Cy5-ssDNA-Biotins were added into streptavi din-conjugated quantum dots in solution, by real time recording, the FRET efficiency was found to increase with time, which indicated the process of streptavidin-conjugated quantum dots capturing Cy5-ssDNA-Biotins. It was also observed that streptavidin-conjugated quantum dots and Cy5-ssDNA-Biotins could both enter living Chinese hamster ovary cells and have FRET. The process of streptavidin-conjugated quantum dots capturing Cy5-ssDNA-Biotins was detected in the cells as well and Cy5s were photobleached after a long time of irradiation. It has been proved that detecting DNA or RNA segments in living cells with DNA nanosensor is possible.

Download full-text PDF

Source

Publication Analysis

Top Keywords

quantum dots
32
dna nanosensor
16
streptavidin-conjugated quantum
16
dna rna
12
rna segments
12
quantum
9
dots
8
dna
8
quantum-dot-based dna
8
capture probes
8

Similar Publications

High-Q Emission from Colloidal Quantum Dots Embedded in Polymer Quasi-BIC Metasurfaces.

Nano Lett

January 2025

Department of Electrical and Computer Engineering, Northwestern University, Evanston, Illinois 60208, United States.

Metasurfaces supporting narrowband resonances are of significant interest in photonics for molecular sensing, quantum light source engineering, and nonlinear photonics. However, many device architectures rely on large refractive index dielectric materials and lengthy fabrication processes. In this work, we demonstrate quasi-bound states in the continuum (quasi-BICs) using a polymer metasurface exhibiting experimental quality factors of 305 at visible wavelengths.

View Article and Find Full Text PDF

Graphene quantum dots (GQDs) are highly valued for their chemical stability, tunable size, and biocompatibility. Utilizing green chemistry, a microwave-assisted synthesis method was employed to produce water-soluble GQDs from Mangifera Indica leaf extract. This approach is efficient, cost-effective, and environmentally friendly, offering reduced reaction times, energy consumption, and uniform particle sizes, and has proven advantageous over other methods.

View Article and Find Full Text PDF

In recent years, despite significant advances in preconcentration and preparation techniques that have led to efficient recovery and accurate measurement of target compounds. There is still a need to develop adsorbents with unique and efficient features such as high pore volume and surface area, reactivity, easy synthesis, low toxicity, and compatibility with the environment, which increase the adsorption capacity and increase extraction efficiency. Semiconductor nanocrystals called quantum dots (QDs) with a size of less than 10 nm are three-dimensional nanoparticles with a spherical, rod, or disc structure that have significant potential in extraction as adsorbents due to their excellent properties such as low toxicity, reactivity, environmental friendliness, and hydrophilic and hydrophobic interactions.

View Article and Find Full Text PDF

Based on nitrogen and phosphorus co-doped carbon dots (NP-CDs), a direct, quick, and selective sensing probe for fluorometric detection of rutin has been developed. Utilizing ethylene diamine tetra acetic acid (EDTA) as a carbon and nitrogen source and diammonium hydrogen phosphate (NH)HPO as a nitrogen and phosphorus source. The NP-CDs were synthesized in less than 3 min with a straightforward one-step microwave pyrolysis process with a high quantum yield (63.

View Article and Find Full Text PDF

Application of advanced quantum dots in perovskite solar cells: synthesis, characterization, mechanism, and performance enhancement.

Mater Horiz

January 2025

Dalian National Laboratory for Clean Energy, iChEM, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.

Quantum dots have garnered significant interest in perovskite solar cells (PSCs) due to their stable chemical properties, high carrier mobility, and unique features such as multiple exciton generation and excellent optoelectronic characteristics resulting from quantum confinement effects. This review explores quantum dot properties and their applications in photoelectronic devices, including their synthesis and deposition processes. This sets the stage for discussing their diverse roles in the carrier transport, absorber, and interfacial layers of PSCs.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!