AI Article Synopsis
- - The study focused on enhancing the production of Cordyceps sinensis using a submerged culture method by experimenting with different carbon sources, primarily rice bran and citrus peel for optimal glucosamine and exopolysaccharide (EPS) yield.
- - The researchers established an optimal nutrient mix (including rice bran, molasses, and other components) and conditions (25°C and 5-6 days culture time) that maximized EPS productivity with significant immune-boosting properties.
- - Adding citrus peel to the culture considerably boosted both EPS and glucosamine content while also increasing anti-complementary (up to 80.8% activity) and antioxidant (AEAC value of 384.6 mg/100g) effects
Article Abstract
To investigate the optimal conditions for the production of Cordyceps sinensis by the submerged culture method, glucosamine and exopolysaccharide (EPS) productivities were determined in culture broth containing different carbon sources, principally rice bran and citrus peel. An optimal medium composition (1.5% rice bran, 0.5% molasses, 3% CSL, 0.1% KH(2)PO(4), and 0.05% MgSO(4)) and the optimal condition (25 degrees C and 5-6 d culture time) for high EPS productivity with potent immune-stimulating activities were obtained. The addition of citrus peel to the culture of C. sinensis under the optimized conditions improved EPS productivity and glucosamine content. Furthermore, anti-complementary activity was higher (58.0-80.8%) using citrus peel as compared to no addition of citrus peel (48.2-68.7%). Antioxidant activity (AEAC value) of the citrus peel culture was high (284.3-384.6 mg/100g) compared to that of the culture without citrus peel (142.8-219.5mg/100g), indicating that the citrus peel helped enhance the anti-complementary and antioxidant activities of C. sinensis.
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| http://dx.doi.org/10.1016/j.biortech.2010.02.083 | DOI Listing |
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