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Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, Türkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA).

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BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting Region.

Pathogens

December 2024

Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

The proviral load (PVL) of the bovine leukemia virus (BLV) is a useful index for estimating disease progression and transmission risk. Real-time quantitative PCR techniques are widely used for PVL quantification. We previously developed a dual-target detection method, the "Liquid Dual-CoCoMo assay", that uses the coordination of common motif (CoCoMo) degenerate primers.

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Background: Bovine leukaemia virus (BLV)-infected Holstein cattle carrying certain bovine leukocyte antigen (BoLA)-DRB3 alleles were previously shown to be resistant to BLV provirus multiplication, while those carrying other alleles were susceptible. This study aimed to determine whether the BoLA-DRB3 alleles carried by BLV-infected cattle could predict proviral load (PVL) and peripheral blood lymphocyte (PBL) count distribution (PVL/PBL distribution).

Methods: Blood samples from Holstein cattle on four dairy farms were tested for the presence of BLV antibodies using a commercial ELISA.

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Biosecurity Practices for Mycobacterium aviun subspecies paratuberculosis Infection, Salmonellosis, and Bovine Leukemia Virus on Cattle Operations.

Vet Clin North Am Food Anim Pract

December 2024

Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Mycobacterium avium subspecies paratuberculosis infection, salmonellosis, and bovine leukosis virus are important dairy and beef cattle diseases from a health perspective. For herds without infection, keeping the disease out through testing of source herds should be prioritized. For herds with infection, control programs should be instituted and followed to reduce the prevalence and ultimately eliminate the disease.

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Article Synopsis
  • The study explores the potential link between Bovine leukemia virus (BLV) infection and breast cancer, responding to ongoing debate and inconsistent previous findings.
  • Researchers conducted a systematic review and meta-analysis, analyzing 17 studies, with 12 included in the final analysis to assess this association and calculate odds ratios.
  • The results indicate a significant statistical association between BLV infection and an increased risk of breast cancer, with a pooled odds ratio suggesting a more than threefold increase in risk when certain studies were excluded for heterogeneity.
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