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Developing a novel TaqMan qPCR assay for optimizing Pullorum detection in chickens.
Vet Q
December 2025
Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Pullorum, enabling precise differentiation from other serovars.
View Article and Find Full Text PDFIsolation, characterization, and genome sequencing analysis of a novel phage HBW-1 of Salmonella.
Microb Pathog
January 2025
Laboratory of Molecular Microbiology and Food Safety, Zhejiang University College of Animal Sciences, Hangzhou, 310058, China; Hainan Institute of Zhejiang University, Sanya, 572025, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China. Electronic address:
Salmonella presents a significant threat to the health of animals and humans, especially with the rise of strains resistant to multiple drugs. This highlights the necessity for creating sustainable and efficient practical approaches to managing salmonellosis. The most recent and safest approach to combat antimicrobial resistance-associated infections is lytic bacteriophages.
View Article and Find Full Text PDFElectrochemiluminescence Resonance Energy Transfer Biosensor for the Human-Associated Clade of Based on Prereduction-Enhanced Yttrium MOFs.
Anal Chem
January 2025
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
, a significant zoonotic pathogen, annually caused substantial economic losses in the swine industry and had intensified threat to public health due to the recent emergence of human-associated clade. In this study, we discovered that the rare-earth metal-based metal-organic frameworks (Y-BTC) possessed excellent ECL capabilities. After prereduction at high voltage, its ECL intensity was enhanced by two times.
View Article and Find Full Text PDFAn enzyme-activated loop primer probe LAMP method based on a new SNP site in the group_17537 gene for rapid on-site detection of Salmonella Pullorum.
Poult Sci
January 2025
National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Key Laboratory of Zoonoses, Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address:
Pullorum disease (PD) is a widespread disease that causes significant economic losses within the poultry industry of developing countries. An effective strategy for its prevention and control involves the implementation of decontamination procedures utilizing highly specific on-site detection techniques. In this study, a single-nucleotide polymorphism (SNP) site within the group_17537 gene of Salmonella enterica serovar Gallinarum biovars Pullorum (S.
View Article and Find Full Text PDFChanges in the intestinal microbiota of broiler chicken induced by dietary supplementation of the diatomite-bentonite mixture.
BMC Vet Res
January 2025
Specialized Mining Company "Górtech" Sp. z o.o, ul. Wielicka 50, Krakow, 30-552, Poland.
Background: Diatomite is a source of biologically available silicon but in feed industry its insecticide and anti-caking properties have been also widely recognized. The aim of the study was to evaluate the effect of dietary diatomite-bentonite mixture (DBM) supplementation on the quantitative and qualitative composition of the bacterial microbiome of the broiler chicken gut. The trial was carried out on 960 Ross 308 broiler chickens divided into 2 experimental groups throughout the entire rearing period lasting 6 weeks.
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