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Optically Actuated Soft Microrobot Family for Single-Cell Manipulation.
Adv Mater
August 2024
HUN-REN Biological Research Centre, Szeged Institute of Biophysics, Temesvári krt. 62, Szeged, 6726, Hungary.
Precisely controlled manipulation of nonadherent single cells is often a pre-requisite for their detailed investigation. Optical trapping provides a versatile means for positioning cells with submicrometer precision or measuring forces with femto-Newton resolution. A variant of the technique, called indirect optical trapping, enables single-cell manipulation with no photodamage and superior spatial control and stability by relying on optically trapped microtools biochemically bound to the cell.
View Article and Find Full Text PDFResearch on Geometric Constraint Strategies for Controlling the Diameter of Micro-Shafts Manufactured via Wire Electric Discharge Grinding.
Micromachines (Basel)
November 2023
Taizhou Jiuju Technology Co., Ltd., Taizhou 317600, China.
Micro-tools comprising difficult-to-machine materials have seen widespread application in micro-manufacturing to satisfy the demands of micro-part processing and micro-device development. Taking micro-shafts as an example, the related developmental technology, based on wire electric discharge grinding (WEDG) as the core method, is one of the key technologies used to prepare high-precision micro-shafts. To enable efficient and high-precision machining of micro-shafts with target diameters, instead of performing multiple repeated on-machine measurements and reprocessing, a geometric constraint strategy is proposed based on the previously introduced twin-mirroring-wire tangential feed electrical discharge grinding (TMTF-WEDG).
View Article and Find Full Text PDFSingle-Cell Elasticity Measurement with an Optically Actuated Microrobot.
Micromachines (Basel)
September 2020
Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary.
A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction.
View Article and Find Full Text PDFFabrication of Fe-based metal glass microelectrodes by a vertical liquid membrane electrochemical etching method.
Rev Sci Instrum
March 2020
College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.
The excellent physical and chemical properties of metallic glasses and the absence of crystal defects make them ideal materials for the preparation of microelectrodes. A vertical liquid membrane electrochemical etching method is proposed here for the fabrication of microelectrodes from the Fe-based metallic glass FeBSi. With this method, insoluble electrolysis products are kept away from the processing area, and the nonuniformity of the diffusion layer that occurs in traditional transverse liquid membrane electrochemical etching is reduced.
View Article and Find Full Text PDFEvaluation of nanoencapsulated verteporfin's cytotoxicity using a microfluidic system.
J Pharm Biomed Anal
August 2016
Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland. Electronic address:
A new-generation of nanoencapsulated photosensitizers could be a good solution to perform effective photodynamic therapy (PDT). In this paper, we present physicochemical characterization and cellular investigation of newly prepared long-sustained release oil-core polyelectrolyte nanocarriers loaded with verteporfin (nano VP) in relation to free VP. For this purpose, a macroscale multiwell plates and multifunctional microfluidic system (for three types of cell cultures: monoculture, coculture and mixed culture) were used.
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