Suppression of water as a nucleophile in Candida antarctica lipase B catalysis.

Chembiochem

Department of Biochemistry, School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, 10691 Stockholm, Sweden.

Published: April 2010

AI Article Synopsis

Article Abstract

A water tunnel in Candida antarctica lipase B that provides the active site with substrate water is hypothesized. A small, focused library created in order to prevent water from entering the active site through the tunnel was screened for increased transacylation over hydrolysis activity. A single mutant, S47L, in which the inner part of the tunnel was blocked, catalysed the transacylation of vinyl butyrate to 20 mM butanol 14 times faster than hydrolysis. The single mutant Q46A, which has a more open outer end of the tunnel, showed an increased hydrolysis rate and a decreased hydrolysis to transacylation ratio compared to the wild-type lipase. Mutants with a blocked tunnel could be very useful in applications in which hydrolysis is unwanted, such as the acylation of highly hydrophilic compounds in the presence of water.

Download full-text PDF

Source
http://dx.doi.org/10.1002/cbic.200900743DOI Listing

Publication Analysis

Top Keywords

candida antarctica
8
antarctica lipase
8
active site
8
single mutant
8
tunnel
5
hydrolysis
5
suppression water
4
water nucleophile
4
nucleophile candida
4
lipase catalysis
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!