Aim: To construct replication-defective HFV-IL24 virus vector and to investigate its inhibitive effect on cancer cells after infected or transfected by this vector.
Methods: pDeltaphi-IL24 was constructed and was co-transfected with helper-plasmids into HEK 293T cells. Recombinant HFV-IL24 vector was extracted to infect HeLa cells and the inhibitive effect of IL-24 on cancer cells was examined by RT-PCR, MTT, cell counting and FCM methods.
Results: pDeltaphi-IL24 plasmid and HFV-IL24 particles were successfully constructed. HeLa cells exhibited obvious growth inhibition, cell cycle variation and visible apoptosis (mortality) after infected or transfected by HFV-IL24 or pDeltaphi-IL24.
Conclusion: The results of the study provide some evidence for human foamy virus used as an effective gene transfer tool and for cancer gene therapy with HFV-IL24.
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