AI Article Synopsis
- The BomaNPV S1 strain is less virulent than the BmNPV T3 strain in infecting the silkworm, Bombyx mori.
- The complete genome of BomaNPV S1, which is 126,770 nucleotides long, was sequenced and found to have high similarity to the BmNPV T3 strain, with 133 potential ORFs identified.
- Differences were noted in specific genes, with BomaNPV S1 lacking the bro-b and bro-e genes and variations observed in repeat units, suggesting that these strains may be variants of each other and highlighting the potential role of genetic differences in baculovirus strain diversity.
Article Abstract
The Bombyx mandarina nucleopolyhedrovirus (BomaNPV) S1 strain can infect the silkworm, Bombyx mori, but is significantly less virulent than B. mori nucleopolyhedrovirus (BmNPV) T3 strain. The complete nucleotide sequence of the S1 strain of BomaNPV was determined and compared with the BmNPV T3 strain. The circular, double stranded DNA genome of the S1 strain was 126,770 nucleotides long (GenBank accession no. FJ882854), with a G+C content of 40.23%. The genome contained 133 potential ORFs. Most of the putative proteins were more than 96% identical to homologs in the BmNPV T3 strain, except for bro-a, lef-12, bro-c, and bro-d. Compared with the BmNPV T3 strain, however, this genome did not encode the bro-b and bro-e genes. In addition, hr1 lacked two repeat units, while hr2L, hr2R, hr3, hr4L, hr4R, and hr5 were similar to the corresponding hrs in the T3 strain. The sequence strongly suggested that BomaNPV and BmNPV are variants with each other, and supported the idea that baculovirus strain heterogeneity may often be caused by variation in the hrs and bro genes.
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| http://dx.doi.org/10.1007/s12275-009-0197-4 | DOI Listing |
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