[Study on the optimal extraction process of chaihushugan powder].

Objective: To study the optimal extraction process of chaihushugan powder by orthogonal design.

Methods: RP-HPLC method was developed for the determination of saikosaponin a, ferulic acid, hesperidin and paeoniflorin in chaihushugan powder. The contents of the components and the extraction yield were selected as assessment indices. Four factors were study by L9 (3(4)), including the alcohol concentration, amount of alcohol, duration of extraction and times of extraction.

Results: The optimal extracting condition was 80% alcohol consumed as 10 times of crude herb amount, and extracting two times for 90 min each time.

Conclusion: This study supplies theoretical base for the development of chaihushugan powder formulation.