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Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes.
Methods Enzymol
June 2021
Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA, United States. Electronic address:
Optogenetic tools allow regulation of cellular processes with light, which can be delivered with spatiotemporal resolution. By combining the chemical versatility of photoremovable protecting groups with the biological specificity of self-labeling tags, we developed a series of chemi-optogenetic tools that enable protein recruitment with precise spatiotemporal control. To this end, we created a modular platform for chemically inducible proximity (CIP), a technique in which two proteins of interest are brought together by the presence of a small molecule to induce a biological effect.
View Article and Find Full Text PDFDesign, synthesis, and application of the trimethoprim-based chemical tag for live-cell imaging.
Curr Protoc Chem Biol
April 2015
Department of Chemistry, Columbia University, New York, New York, USA.
Over the past decade, chemical tags have been developed to complement the use of fluorescent proteins in live-cell imaging. Chemical tags retain the specificity of protein labeling achieved with fluorescent proteins through genetic encoding, but provide smaller, more robust tags and modular use of organic fluorophores with high photon output and tailored functionalities. The trimethoprim-based chemical tag (TMP-tag) was initially developed based on the high affinity interaction between E.
View Article and Find Full Text PDFA fluorogenic TMP-tag for high signal-to-background intracellular live cell imaging.
ACS Chem Biol
August 2013
Department of Chemistry, Columbia University, 550 West 120th Street, MC 4854, NWC Building, New York, New York 10027, United States.
Developed to complement the use of fluorescent proteins in live cell imaging, chemical tags enjoy the benefit of modular incorporation of organic fluorophores, opening the possibility of high photon output and special photophysical properties. However, the theoretical challenge in using chemical tags as opposed to fluorescent proteins for high-resolution imaging is background noise from unbound and/or nonspecifically bound ligand-fluorophore. We envisioned we could overcome this limit by engineering fluorogenic trimethoprim-based chemical tags (TMP-tags) in which the fluorophore is quenched until binding with E.
View Article and Find Full Text PDFA trimethoprim-based chemical tag for live cell two-photon imaging.
Chembiochem
April 2010
Department of Chemistry, Columbia University, 3000 Broadway, MC 3111, New York, NY 10027, USA.
Neural tube defects in relation to use of folic acid antagonists during pregnancy.
Am J Epidemiol
May 2001
Slone Epidemiology Unit, Boston University School of Public Health, Brookline, MA 02446, USA.
Periconceptional folic acid supplementation reduces the risk of neural tube defects (NTDs). To determine whether periconceptional exposure to folic acid antagonists (FAAs) might therefore increase the risk of NTDs, the authors examined data from an ongoing case-control study of birth defects (1979-1998) in the United States and Canada. They compared data on 1,242 infants with NTDs (spina bifida, anencephaly, and encephalocele) with data from a control group of 6,660 infants with malformations not related to vitamin supplementation.
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