Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860026 | PMC |
| http://dx.doi.org/10.1016/j.ab.2010.03.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Venom composition, toxicity and cross-neutralization by PoliVal-ICP antivenom, of Mesoamerican jumping pitvipers genus Metlapilcoatlus (Viperidae: Crotalinae).
Trans R Soc Trop Med Hyg
January 2025
Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José 11501, Costa Rica.
Background: The genus Metlapilcoatlus was recently erected to include six species of stout venomous snakes, known as the jumping pitvipers, which inhabit mountainous areas of Mesoamerica. This group maintains affinity with Atropoides picadoi, another jumping pitviper with restricted distribution in Costa Rica and Panama. Although the venom of A.
View Article and Find Full Text PDFDevelopment and thorough evaluation of a multi-omics sample preparation workflow for comprehensive LC-MS/MS-based metabolomics, lipidomics and proteomics datasets.
Talanta
December 2024
Hyphenated Mass Spectrometry Laboratory, Faculty of Science, University of Technology Sydney, PO Box 123, Broadway, 2007 NSW, Australia; School of Life Sciences, Faculty of Science, University of Technology Sydney, PO Box 123, Broadway, 2007 NSW, Australia.
The importance of sample preparation selection if often overlooked particularly for untargeted multi-omics approaches that gained popularity in recent years. To minimize issues with sample heterogeneity and additional freeze-thaw cycles during sample splitting, multiple -omics datasets (e.g.
View Article and Find Full Text PDFStructural insights into how Cas9 targets nucleosomes.
Nat Commun
December 2024
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
The CRISPR-associated endonuclease Cas9 derived from prokaryotes is used as a genome editing, which targets specific genomic loci by single guide RNAs (sgRNAs). The eukaryotes, the target of genome editing, store their genome DNA in chromatin, in which the nucleosome is a basic unit. Despite previous structural analyses focusing on Cas9 cleaving free DNA, structural insights into Cas9 targeting of DNA within nucleosomes are limited, leading to uncertainties in understanding how Cas9 operates in the eukaryotic genome.
View Article and Find Full Text PDFBreaking the Ice: Hofmeister Effect-Inspired Hydrogen Bond Network Reconstruction in Hydrogel Electrolytes for High-Performance Zinc-Ion Batteries.
Small
December 2024
State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
Gel electrolytes have emerged as a promising solution for enhancing the performance of zinc-ion batteries (ZIBs), particularly in flexible devices. However, they face challenges such as low-temperature inefficiency, constrained ionic conductivity, and poor mechanical strength. To address these issues, this study presents a novel PAMCD gel electrolyte with tunable freezing point and mechanical properties for ZIBs, blending the high ionic conductivity of polyacrylamide with the anion interaction capability of β-cyclodextrin.
View Article and Find Full Text PDFRevealing O-acetylhomoserine sulfhydrylase involved in direct sulfhydrylation pathway in Clostridium tetani.
Biochimie
December 2024
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, 32 Vavilov St., Moscow 119991, Russia.
Bacterial methionine biosynthesis is an attractive target for research due to its central role in cellular metabolism, as most steps of this pathway are missing in mammals. Up to now little is known about sulfur metabolism in pathogenic Clostridia species, making the study of the enzymes of Cys/Met metabolism in Clostridium tetani particularly relevant. Analysis of the C.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!