We propose a new, simple theory-based, accurate polarization microscope for birefringence imaging of cytoskeletal structures of biological cells. The new theory lets us calculate very easily the phase retardation and the orientation of the principal axis of a particular area of a biological living cell in media by simply measuring the intensity variation of a pixel of a CCD camera while rotating a single polarizer. Just from the measured intensity maxima and minima, the amount of phase retardation delta between the fast and the slow axis of the sample area is obtained with an accuracy of 5.010+/-0.798x10(-3) rad. The orientation of the principal axis is calculated from the angle of the polarizer for the intensity maximum. We have compared our microscopes with two previously reported polarization microscopes for birefringence imaging of cytoskeletal structures and demonstrated the utility of our microscope with the phase retardation and orientation images of weakly invasive MCF7 and highly invasive MDA MB 231 human breast cancer cells as an example.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1117/1.3327280 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthetic polarization-sensitive optical coherence tomography using contrastive unpaired translation.
Sci Rep
December 2024
Department of Artificial Intelligence Convergence, Chonnam National University, Gwangju, 61186, Republic of Korea.
Polarization-sensitive optical coherence tomography (PS-OCT) measures the polarization state of backscattered light from tissues and provides valuable insights into the birefringence properties of biological tissues. Contrastive unpaired translation (CUT) was used in this study to generate a synthetic PS-OCT image from a single OCT image. The challenges related to extensive data requirements relying on labeled datasets using only pixel-wise correlations that make it difficult to efficiently regenerate the periodic patterns observed in PS-OCT images were addressed.
View Article and Find Full Text PDFFilm thickness dependence of nanoscale arrangement of a chiral electron donor in its blends with an achiral electron acceptor.
Nanoscale
December 2024
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Carrer dels Til·lers, Bellaterra, 08193, Spain.
The nanoscale chiral arrangement in a bicomponent organic material system comprising donor and acceptor small molecules is shown to depend on the thickness of a film that is responsive to chiral light in an optoelectronic device. In this bulk heterojunction, a previously unreported chiral bis(diketopyrrolopyrrole) derivative was combined with an achiral non-fullerene acceptor. The optical activity of the chiral compound is dramatically different in the pure material and the composite, showing how the electron acceptor influences the donor's arrangement compared with the pure molecule.
View Article and Find Full Text PDFAbsolute depth-resolved optic axis measurement with catheter-based polarization sensitive optical coherence tomography.
Biomed Opt Express
December 2024
Harvard Medical School, Boston, MA, USA.
Imaging depth-resolved birefringence and optic axis orientation with polarization sensitive optical coherence tomography (PS-OCT) unveils details of tissue structure and organization that can be of high pathophysiologic, mechanistic, and diagnostic value. For catheter-based PS-OCT, the dynamic rotation of the fiber optic probe, in addition to the polarization effects of the system components, complicates the reliable and robust reconstruction of the sample's optic axis orientation. Addressing this issue, we present a new method for the reconstruction of absolute depth-resolved optic axis orientation in catheter-based PS-OCT by using the intrinsic retardance of the protecting catheter sheath as a stable guide star signal.
View Article and Find Full Text PDFTransverse modulation instability (MI) has been proved useful for reconstructing noisy images. However, the signal-noise resonances for high-frequency modes are always suppressed during the generation of instability, resulting in the blurring of output images. By controlling of photo-birefringence and isomerization of azobenzene-derivative polymer, we proposed an instability-driven reconstruction by re-growing high-frequency modes via localizing wave response.
View Article and Find Full Text PDFMueller matrix polarimetry for quantitative evaluation of the Achilles tendon injury recovery.
Front Optoelectron
December 2024
Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Achilles tendon injuries, as a widely existing disease, have attracted a lot of research interest. Mueller matrix polarimetry, as a novel label-free quantitative imaging method, has been widely used in various applications of lesion identification and pathological diagnosis. However, focusing on the recovery process of Achilles tendon injuries, current optical imaging methods have not yet achieved the label-free precise identification and quantitative evaluation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!