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1875-9777632010Mar05Cell stem cellCell Stem CellEndothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells.251264251-6410.1016/j.stem.2010.02.001Bone marrow endothelial cells (ECs) are essential for reconstitution of hematopoiesis, but their role in self-renewal of long-term hematopoietic stem cells (LT-HSCs) is unknown. We have developed angiogenic models to demonstrate that EC-derived angiocrine growth factors support in vitro self-renewal and in vivo repopulation of authentic LT-HSCs. In serum/cytokine-free cocultures, ECs, through direct cellular contact, stimulated incremental expansion of repopulating CD34(-)Flt3(-)cKit(+)Lineage(-)Sca1(+) LT-HSCs, which retained their self-renewal ability, as determined by single-cell and serial transplantation assays. Angiocrine expression of Notch ligands by ECs promoted proliferation and prevented exhaustion of LT-HSCs derived from wild-type, but not Notch1/Notch2-deficient, mice. In transgenic notch-reporter (TNR.Gfp) mice, regenerating TNR.Gfp(+) LT-HSCs were detected in cellular contact with sinusoidal ECs. Interference with angiocrine, but not perfusion, function of SECs impaired repopulation of TNR.Gfp(+) LT-HSCs. ECs establish an instructive vascular niche for clinical-scale expansion of LT-HSCs and a cellular platform to identify stem cell-active trophogens.2010 Elsevier Inc. All rights reserved.ButlerJason MJMHoward Hughes Medical Institute, Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10065, USA.NolanDaniel JDJVertesEva LELVarnum-FinneyBarbaraBKobayashiHidekiHHooperAndrea TATSeandelMarcoMShidoKojiKWhiteIan AIAKobayashiMarikoMWitteLarryLMayChadCShawberCarrieCKimuraYukiYKitajewskiJanJRosenwaksZevZBernsteinIrwin DIDRafiiShahinSengRC1 AI080309-01AINIAID NIH HHSUnited StatesP01 HL059312-090006HLNHLBI NIH HHSUnited StatesR01 HL097797-03HLNHLBI NIH HHSUnited StatesP50 HL084936HLNHLBI NIH HHSUnited StatesP01 HL059312-100006HLNHLBI NIH HHSUnited StatesR01 HL097797-01HLNHLBI NIH HHSUnited StatesU01 HL066952-020002HLNHLBI NIH HHSUnited StatesRC1 AI080309AINIAID NIH HHSUnited StatesP01 HL059312HLNHLBI NIH HHSUnited StatesU01 HL066952-040002HLNHLBI NIH HHSUnited StatesP01 HL059312-080006HLNHLBI NIH HHSUnited StatesHL075234HLNHLBI NIH HHSUnited StatesR21 HL083222-01HLNHLBI NIH HHSUnited StatesU01 HL066952HLNHLBI NIH HHSUnited StatesU01 HL066952-030002HLNHLBI NIH HHSUnited StatesP50 HL084936-010003HLNHLBI NIH HHSUnited StatesR21 HL083222-02HLNHLBI NIH HHSUnited StatesP01 HL067839HLNHLBI NIH HHSUnited StatesP50 HL084936-030003HLNHLBI NIH HHSUnited StatesR01 HL097797-02HLNHLBI NIH HHSUnited StatesR01 HL097797HLNHLBI NIH HHSUnited StatesP50 HL084936-020003HLNHLBI NIH HHSUnited StatesP01 HL059312-060006HLNHLBI NIH HHSUnited StatesP01 HL084205HLNHLBI NIH HHSUnited StatesHL097797HLNHLBI NIH HHSUnited StatesP01 HL067839-050004HLNHLBI NIH HHSUnited StatesP01 HL059312-070006HLNHLBI NIH HHSUnited StatesP50 HL084936-040003HLNHLBI NIH HHSUnited StatesU01 HL066952-050002HLNHLBI NIH HHSUnited StatesU01 HL066952-010002HLNHLBI NIH HHSUnited StatesR01 CA136673CANCI NIH HHSUnited StatesHHMIHoward Hughes Medical InstituteUnited StatesP01 HL067839-040004HLNHLBI NIH HHSUnited StatesR21 HL083222HLNHLBI NIH HHSUnited StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't
United StatesCell Stem Cell1013114721875-97770Culture Media, Conditioned0Ligands0Notch1 protein, mouse0Notch2 protein, mouse0Receptor, Notch10Receptor, Notch2IMAnimalsCell CommunicationCell LineageCell ProliferationCells, CulturedCoculture TechniquesCulture Media, ConditionedEndothelial CellscytologymetabolismHematopoietic Stem CellscytologymetabolismLigandsMiceMice, KnockoutReceptor, Notch1deficiencymetabolismReceptor, Notch2deficiencymetabolismSignal Transduction200810920099212010232010396020103960201033060201095ppublish20207228NIHMS186877PMC286652710.1016/j.stem.2010.02.001S1934-5909(10)00045-7Antonchuk J, Sauvageau G, Humphries RK. HOXB4-induced expansion of adult hematopoietic stem cells ex vivo. Cell. 2002;109:39–45.11955445Arai F, Hirao A, Ohmura M, Sato H, Matsuoka S, Takubo K, Ito K, Koh GY, Suda T. Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell. 2004;118:149–161.15260986Avecilla ST, Hattori K, Heissig B, Tejada R, Liao F, Shido K, Jin DK, Dias S, Zhang F, Hartman TE, et al. Chemokine-mediated interaction of hematopoietic progenitors with the bone marrow vascular niche is required for thrombopoiesis. Nat Med. 2004;10:64–71.14702636Butler JM, Kobayahsi H, Rafii S. Instructive role of the vascular niche in promoting tumour growth and tissue repair by angiocrine factors. Nat Rev Cancer. 2010;10(2):138–46.PMC294477520094048Calvi LM, Adams GB, Weibrecht KW, Weber JM, Olson DP, Knight MC, Martin RP, Schipani E, Divieti P, Bringhurst FR, et al. Osteoblastic cells regulate the haematopoietic stem cell niche. Nature. 2003;425:841–846.14574413Carmeliet P, Lampugnani MG, Moons L, Breviario F, Compernolle V, Bono F, Balconi G, Spagnuolo R, Oostuyse B, Dewerchin M, et al. Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell. 1999;98:147–157.10428027Duncan AW, Rattis FM, DiMascio LN, Congdon KL, Pazianos G, Zhao C, Yoon K, Cook JM, Willert K, Gaiano N, et al. Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Nat Immunol. 2005;6:314–322.15665828Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, et al. Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation. Exp Hematol. 2008;36:545–558.PMC343776018439488Funahashi Y, Hernandez SL, Das I, Ahn A, Huang J, Vorontchikhina M, Sharma A, Kanamaru E, Borisenko V, Desilva DM, et al. A notch1 ectodomain construct inhibits endothelial notch signaling, tumor growth, and angiogenesis. Cancer Res. 2008;68:4727–4735.PMC369060218559519Gerber HP, Malik AK, Solar GP, Sherman D, Liang XH, Meng G, Hong K, Marsters JC, Ferrara N. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Nature. 2002;417:954–958.12087404Hadland BK, Huppert SS, Kanungo J, Xue Y, Jiang R, Gridley T, Conlon RA, Cheng AM, Kopan R, Longmore GD. A requirement for Notch1 distinguishes 2 phases of definitive hematopoiesis during development. Blood. 2004;104:3097–3105.PMC599865915251982Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, et al. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell. 2002;109:625–637.PMC282611012062105Hooper AT, Butler JM, Nolan DJ, Kranz A, Iida K, Kobayashi M, Kopp HG, Shido K, Petit I, Yanger K, et al. Engraftment and reconstitution of hematopoiesis is dependent on VEGFR2-mediated regeneration of sinusoidal endothelial cells. Cell Stem Cell. 2009;4:263–274.PMC322827519265665Karanu FN, Murdoch B, Gallacher L, Wu DM, Koremoto M, Sakano S, Bhatia M. The notch ligand jagged-1 represents a novel growth factor of human hematopoietic stem cells. J Exp Med. 2000;192:1365–1372.PMC219335211067884Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005;121:1109–1121.15989959Kollet O, Dar A, Shivtiel S, Kalinkovich A, Lapid K, Sztainberg Y, Tesio M, Samstein RM, Goichberg P, Spiegel A, et al. Osteoclasts degrade endosteal components and promote mobilization of hematopoietic progenitor cells. Nat Med. 2006;12:657–664.16715089Kopp HG, Hooper AT, Broekman MJ, Avecilla ST, Petit I, Luo M, Milde T, Ramos CA, Zhang F, Kopp T, et al. Thrombospondins deployed by thrombopoietic cells determine angiogenic switch and extent of revascularization. J Clin Invest. 2006;116:3277–3291.PMC167971017143334Krosl J, Austin P, Beslu N, Kroon E, Humphries RK, Sauvageau G. In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein. Nat Med. 2003;9:1428–1432.14578881Maillard I, Koch U, Dumortier A, Shestova O, Xu L, Sai H, Pross SE, Aster JC, Bhandoola A, Radtke F, et al. Canonical notch signaling is dispensable for the maintenance of adult hematopoietic stem cells. Cell Stem Cell. 2008;2:356–366.PMC371737318397755Mancini SJ, Mantei N, Dumortier A, Suter U, MacDonald HR, Radtke F. Jagged1-dependent Notch signaling is dispensable for hematopoietic stem cell self-renewal and differentiation. Blood. 2005;105:2340–2342.15550486Miller CL, Eaves CJ. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability. Proc Natl Acad Sci U S A. 1997;94:13648–13653.PMC283609391080Mizutani K, Yoon K, Dang L, Tokunaga A, Gaiano N. Differential Notch signalling distinguishes neural stem cells from intermediate progenitors. Nature. 2007;449:351–355.17721509Rafii S, Shapiro F, Pettengell R, Ferris B, Nachman RL, Moore MA, Asch AS. Human bone marrow microvascular endothelial cells support long-term proliferation and differentiation of myeloid and megakaryocytic progenitors. Blood. 1995;86:3353–3363.7579438Ramalingam R, Rafii S, Worgall S, Brough DE, Crystal RG. E1(-)E4(+) adenoviral gene transfer vectors function as a “pro-life” signal to promote survival of primary human endothelial cells. Blood. 1999;93:2936–2944.10216088Seandel M, Butler JM, Kobayashi H, Hooper AT, White IA, Zhang F, Vertes EL, Kobayashi M, Zhang Y, Shmelkov SV, et al. Generation of a functional and durable vascular niche by the adenoviral E4ORF1 gene. Proc Natl Acad Sci U S A. 2008;105:19288–19293.PMC258841419036927Till JE, McCulloch EA, Siminovitch L. A Stochastic Model of Stem Cell Proliferation, Based on the Growth of Spleen Colony-Forming Cells. Proc Natl Acad Sci U S A. 1964;51:29–36.PMC30059914104600Varnum-Finney B, Xu L, Brashem-Stein C, Nourigat C, Flowers D, Bakkour S, Pear WS, Bernstein ID. Pluripotent, cytokine-dependent, hematopoietic stem cells are immortalized by constitutive Notch1 signaling. Nat Med. 2000;6:1278–1281.11062542Willert K, Brown JD, Danenberg E, Duncan AW, Weissman IL, Reya T, Yates JR, 3rd, Nusse R. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature. 2003;423:448–452.12717451Wolfe MS, Xia W, Moore CL, Leatherwood DD, Ostaszewski B, Rahmati T, Donkor IO, Selkoe DJ. Peptidomimetic probes and molecular modeling suggest that Alzheimer's gamma-secretase is an intramembrane-cleaving aspartyl protease. Biochemistry. 1999;38:4720–4727.10200159Wu M, Kwon HY, Rattis F, Blum J, Zhao C, Ashkenazi R, Jackson TL, Gaiano N, Oliver T, Reya T. Imaging hematopoietic precursor division in real time. Cell Stem Cell. 2007;1:541–554.PMC226776218345353Zhang CC, Kaba M, Ge G, Xie K, Tong W, Hug C, Lodish HF. Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells. Nat Med. 2006;12:240–245.PMC277141216429146Zhang F, Cheng J, Hackett NR, Lam G, Shido K, Pergolizzi R, Jin DK, Crystal RG, Rafii S. Adenovirus E4 gene promotes selective endothelial cell survival and angiogenesis via activation of the vascular endothelial-cadherin/Akt signaling pathway. J Biol Chem. 2004;279:11760–11766.14660586Zhang F, Cheng J, Lam G, Jin DK, Vincent L, Hackett NR, Wang S, Young LM, Hempstead B, Crystal RG, et al. Adenovirus vector E4 gene regulates connexin 40 and 43 expression in endothelial cells via PKA and PI3K signal pathways. Circ Res. 2005;96:950–957.PMC293519815831817Zhang J, Niu C, Ye L, Huang H, He X, Tong WG, Ross J, Haug J, Johnson T, Feng JQ, et al. Identification of the haematopoietic stem cell niche and control of the niche size. Nature. 2003;425:836–841.14574412trying2...
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Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells. | LitMetric
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Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells.

Jason M Butler Daniel J Nolan Eva L Vertes Barbara Varnum-Finney Hideki Kobayashi Andrea T Hooper Marco Seandel Koji Shido Ian A White Mariko Kobayashi Larry Witte Chad May Carrie Shawber Yuki Kimura Jan Kitajewski Zev Rosenwaks Irwin D Bernstein Shahin Rafii

Cell Stem Cell

Howard Hughes Medical Institute, Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10065, USA.

Published: March 2010

Bone marrow endothelial cells (ECs) are essential for reconstitution of hematopoiesis, but their role in self-renewal of long-term hematopoietic stem cells (LT-HSCs) is unknown. We have developed angiogenic models to demonstrate that EC-derived angiocrine growth factors support in vitro self-renewal and in vivo repopulation of authentic LT-HSCs. In serum/cytokine-free cocultures, ECs, through direct cellular contact, stimulated incremental expansion of repopulating CD34(-)Flt3(-)cKit(+)Lineage(-)Sca1(+) LT-HSCs, which retained their self-renewal ability, as determined by single-cell and serial transplantation assays. Angiocrine expression of Notch ligands by ECs promoted proliferation and prevented exhaustion of LT-HSCs derived from wild-type, but not Notch1/Notch2-deficient, mice. In transgenic notch-reporter (TNR.Gfp) mice, regenerating TNR.Gfp(+) LT-HSCs were detected in cellular contact with sinusoidal ECs. Interference with angiocrine, but not perfusion, function of SECs impaired repopulation of TNR.Gfp(+) LT-HSCs. ECs establish an instructive vascular niche for clinical-scale expansion of LT-HSCs and a cellular platform to identify stem cell-active trophogens.

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