AI Article Synopsis
- A method for separating alpha- from beta-arylalanines using ligand exchange chromatography on a nickel nitrilotriacetate agarose column is described, utilizing UV monitoring for effluent detection.
- Each mixture containing 1 mg of alpha- and beta-arylalanines was loaded onto a pre-equilibrated nickel resin and eluted with a gradient of pH 12.0 to 8.0, with beta-arylalanines eluting before alpha-isomers.
- The process achieved baseline resolution for all tested pairs, with over 98% sample recovery and strong resilience of the column, maintaining consistent separation over approximately 100 cycles.
Article Abstract
A method is described to separate alpha- from beta-arylalanines by ligand exchange chromatography on a nickel nitrilotriacetate agarose column with UV monitoring of the effluent. Separate mixtures containing an alpha- and beta-arylalanine pair (1 mg of each) were individually loaded onto the nickel resin pre-equilibrated with the mobile phase at room temperature, and the amino acids were eluted from the column with a gradient from pH 12.0-8.0. The beta-arylalanines eluted first, followed by the alpha-isomers. The four alpha/beta-amino acid pairs tested were well separated with baseline resolution. An aliquot of each fraction was chemically treated to derivatize the amino acids to their N-acyl methyl ester analogs, and their identities were confirmed by GC/MS analysis. The sample recovery was quantitative (>98%), and the column matrix was very resilient, as demonstrated by consistent separation of the solutes after approximately 100 preparative cycles.
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| http://dx.doi.org/10.1002/jssc.200900675 | DOI Listing |
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