Primary transformants of SR1 Nicotiana tabacum plants with RNA interference-based silencing of the gene for extracellular ribonuclease Nk1 were constructed. It was demonstrated that the profiles of ribonuclease activities of leaf protein extracts from these plants lacked ribonuclease with electrophoretic mobility corresponding to that of the Nk1 protein. Primary transformants did not differ phenotypically from control plants. They represent a new model for investigation of the biological role of extracellular ribonucleases, including the molecular mechanisms of resistance to pathogens.
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