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Dendritic effects of genetically encoded actin-labeling probes in cultured hippocampal neurons.
Mol Biol Cell
June 2023
Department of Physiology and Neurobiology, Eötvös Loránd University, Budapest, Hungary.
Actin cytoskeleton predominantly regulates the formation and maintenance of synapses by controlling dendritic spine morphology and motility. To visualize actin dynamics, actin molecules can be labeled by genetically fusing fluorescent proteins to actin monomers, actin-binding proteins, or single-chain anti-actin antibodies. In the present study, we compared the dendritic effect of EGFP-actin, LifeAct-TagGFP2 (LifeAct-GFP), and Actin-Chromobody-TagGFP2 (AC-GFP) in mouse cultured hippocampal neurons using unbiased quantitative methods.
View Article and Find Full Text PDFLoss of secreted gelsolin enhances response to anticancer therapies.
J Immunother Cancer
September 2022
Immunobiology Laboratory, The Francis Crick Institute, London, UK
Type 1 conventional dendritic cells (cDC1) play a critical role in priming anticancer cytotoxic CD8 T cells. DNGR-1 (a.k.
View Article and Find Full Text PDFRe-evaluating the actin-dependence of spectraplakin functions during axon growth and maintenance.
Dev Neurobiol
May 2022
Manchester Academic Health Science Centre, Faculty of Biology, Medicine and Health, School of Biology, The University of Manchester, Manchester, UK.
Axons are the long and slender processes of neurons constituting the biological cables that wire the nervous system. The growth and maintenance of axons require loose microtubule bundles that extend through their entire length. Understanding microtubule regulation is therefore an essential aspect of axon biology.
View Article and Find Full Text PDFComparative Studies of Renin-Null Zebrafish and Mice Provide New Functional Insights.
Hypertension
March 2022
Centre for Cardiovascular Science (S.H., L.M., S.R., C.B., C.B.B., A.A., Z.L., J.M.), The Queen's Medical Research Institute, The University of Edinburgh, United Kingdom.
Background: The renin-angiotensin system is highly conserved across vertebrates, including zebrafish, which possess orthologous genes coding for renin-angiotensin system proteins, and specialized mural cells of the kidney arterioles, capable of synthesising and secreting renin.
Methods: We generated zebrafish with CRISPR-Cas9-targeted knockout of renin () to investigate renin function in a low blood pressure environment. We used single-cell (10×) RNA sequencing analysis to compare the transcriptome profiles of renin lineage cells from mesonephric kidneys of with zebrafish and with the metanephric kidneys of and mice.
WASp Is Crucial for the Unique Architecture of the Immunological Synapse in Germinal Center B-Cells.
Front Cell Dev Biol
June 2021
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, College Park, MD, United States.
B-cells undergo somatic hypermutation and affinity maturation in germinal centers. Somatic hypermutated germinal center B-cells (GCBs) compete to engage with and capture antigens on follicular dendritic cells. Recent studies show that when encountering membrane antigens, GCBs generate actin-rich pod-like structures with B-cell receptor (BCR) microclusters to facilitate affinity discrimination.
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