AI Article Synopsis
- The classical chromosome-banding techniques for mammalian chromosomes can't effectively distinguish euchromatic arms in Drosophila mitotic chromosomes.
- Certain techniques, like quinacrine, Hoechst, and N-banding, do allow for clear banding of Drosophila heterochromatin, identifying 61 specific cytological entities.
- The article offers protocols for chromosome banding, detailing methods that use Hoechst, DAPI, quinacrine, and Giemsa stains, which are effective for various Drosophila and mosquito species.
Article Abstract
The classical chromosome-banding techniques developed for mammalian chromosomes do not differentiate the euchromatic arms of Drosophila mitotic chromosomes. However, some of these techniques produce a sharp and highly reproducible banding of Drosophila heterochromatin. For example, the use of quinacrine-, Hoechst-, and N-banding differentiates Drosophila heterochromatin into 61 cytological entities, allowing precise localization of heterochromatic breakpoints. These banding techniques can also be successfully used to differentiate mitotic heterochromatin of various Drosophila and mosquito species. Here we present protocols routinely used in our laboratories for chromosome banding, including the use of Hoechst, 4',6-diamidino-2-phenylindole (DAPI), quinacrine, and Giemsa stains.
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| http://dx.doi.org/10.1101/pdb.prot5390 | DOI Listing |
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