Clenbuterol, a Beta(2)-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino acids) and found to be the pig homolog of large tumor suppressor 2 (Lats2). We mapped pig Lats2 to chromosome 11p13-14 by using FISH, and western blotting demonstrated that pig Lats2 protein was most abundant in adipose. In Drosophila, Lats2 ortholog was reported as a key component of the Hippo pathway which regulates cell differentiation and growth. Here, we show that pig Lats2 exhibit inverted expression to YAP1, another member of the Hippo pathway which positively regulates cell growth and proliferation, during the differentiation of 3T3-L1 preadipocytes. Our results suggested that Lats2 may involve in Hippo pathway regulating the fat reduction by inhibiting adipocyte differentiation and growth.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5483/bmbrep.2010.43.2.097 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative transcriptomic analysis revealed potential mechanisms regulating the hypertrophy of goose pectoral muscles.
Poult Sci
December 2024
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China. Electronic address:
Pectoral muscle development is an important economic trait. According to the different essence, muscle development can be divided into 2 processes: embryonic muscle fiber generation and postnatal muscle fiber hypertrophy, and postnatal muscle fiber hypertrophy has a greater impact on muscle development than the number of muscle fibers formed during the embryonic phase in poultry. However, the underlying mechanisms regulating the hypertrophy of goose pectoral muscles have not been elucidated.
View Article and Find Full Text PDFNeurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development.
Front Cell Dev Biol
July 2023
Veterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of Korea.
Neurotrophin-4 (NT-4), a neurotrophic factor, appears to affect early embryonic development because it is secreted not only by neurons but also by oviductal and uterine epithelial cells. However, no studies have characterized the effects of NT-4 on early embryonic development in pigs. In this study, we applied the experimental model of parthenogenetic-activation (PA)-derived embryos.
View Article and Find Full Text PDFEffect of Downregulating the Hippo Pathway Members YAP1 and LATS2 Transcripts on Early Development and Gene Expression Involved in Differentiation in Porcine Embryos.
Cell Reprogram
April 2020
The United Graduate School of Agricultural Sciences, Iwate University, Morioka, Japan.
Article Synopsis
- The Hippo pathway plays a crucial role in the differentiation of the inner cell mass (ICM) and trophectoderm (TE) during early mouse embryonic development, but its function in pigs (porcine embryogenesis) had not been studied before this research.
- Researchers analyzed the gene expression of Hippo pathway components, YAP1 and LATS2, during porcine preimplantation development and found that their levels were high in matured oocytes and 1-cell embryos but decreased as development progressed.
- The study showed that downregulation of YAP1 or LATS2 hindered preimplantation development and altered the expression of key transcription factors needed for ICM and TE differentiation, suggesting the Hippo pathway is vital
Pig large tumor suppressor 2 (Lats2), a novel gene that may regulate the fat reduction in adipocyte.
BMB Rep
February 2010
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
Clenbuterol, a Beta(2)-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino acids) and found to be the pig homolog of large tumor suppressor 2 (Lats2). We mapped pig Lats2 to chromosome 11p13-14 by using FISH, and western blotting demonstrated that pig Lats2 protein was most abundant in adipose.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!