Purpose: To assess the retention of glass FRC posts luted with resin cements after conditioning the canal dentin with different acidic treatments.
Methods: Post spaces were prepared in six groups of eight endodontically treated extracted teeth. Glass FRC posts were luted after etching the canal dentin with phosphoric acid and/or applying ED-Primer, Clearfil New Bond or XP Bond. The groups with their respective etching time, primer and cement combinations were as follows. ED: ED-Primer only and Panavia 21; ED15: 15 seconds phosphoric acid treatment, ED-Primer and Panavia 21; NB15: 15 seconds phosphoric acid treatment, Clearfil New Bond and Panavia 21; NB30: 30 seconds phosphoric acid treatment, Clearfil New Bond and Panavia 21; XP15: 15 seconds phosphoric acid treatment, XP Bond and Calibra; XP30: 30 seconds phosphoric acid treatment, XP Bond and Calibra. Specimens were stored in water for 30 days and subjected to simulated aging conditions. Post retention was measured in tension at a crosshead speed of 2 mm/minute. Analysis of variance and Bonferroni multiple range tests were conducted for data analysis.
Results: The mean (standard deviation) retention values in Newtons (N) were: ED: 180.9 (44.9); ED15: 278.3 (37.7); NB15: 283.8 (84.9); NB30: 187.7 (60.0), XP15: 216.8 (46.8), XP30: 199.6 (34.0). The retention values of ED15 and NB15 were significantly higher than those of ED and NB30 groups respectively. There were no statistically significant differences between XP Bond groups. Microscopic evaluation of post surfaces demonstrated that the debonding failure was adhesive at the cement-post and cement-dentin interfaces.
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J Orthop Surg Res
January 2025
Xuzhou Medical University Affiliated Stomatology Hospital, Xuzhou, 221002, Jiangsu Province, China.
Purpose: We aimed to explore the mechanism by which Boron-doped nano-hydroxyapatite (B-nHAp) facilitates the proliferation and differentiation of osteoblasts through controlled release of B.
Methods: B-nHAp characterization was accomplished by means of X-ray diffraction, scanning electron microscopy, inductively coupled plasma mass spectrometry, and transmission electron microscopy. Human bone marrow mesenchymal stem cells (hBMSCs) were subjected to flow cytometry, alizarin red S staining, and cell counting kit-8 assay for proliferation and differentiation determination.
Nat Commun
January 2025
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, China.
Accidental ingestion of lead (Pb)-contaminated soils represents a major route of Pb exposure for both adults and children, and the development of accessible and cost-effective solutions to reduce Pb poisoning is urgently required. Here, we present an effective and straightforward technique, involving the consumption of cola beverages, for the purpose of lowering blood Pb levels following the ingestion of contaminated soils in animal models. This method facilitated the direct passage of Pb in contaminated soil through the digestive system, enhancing its elimination without absorption into systemic circulation.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Biochemistry and Structural Biology, UT Health San Antonio, San Antonio, TX, USA.
SAMHD1 is a dNTPase that impedes replication of HIV-1 in myeloid cells and resting T lymphocytes. Here we elucidate the substrate activation mechanism of SAMHD1, which involves dNTP binding at allosteric sites and transient tetramerization. Our findings reveal that tetramerization alone is insufficient to promote dNTP hydrolysis; instead, the activation mechanism requires an inactive tetrameric intermediate with partially occupied allosteric sites.
View Article and Find Full Text PDFInt J Paediatr Dent
January 2025
Department of Paediatric Dentistry, Medical Centre for Dentistry, University Medical Centre Giessen and Marburg GmbH (Campus Giessen), Justus-Liebig-University, Giessen, Germany.
Background: Limited reports are available regarding bonding of universal adhesives to primary teeth' enamel.
Aim: To evaluate the effect of application mode and aging on microtensile bond strength (μTBS) of universal adhesives to primary enamel.
Design: Ninety-six human primary molars were randomly assigned to three groups: SU: Scotchbond Universal (3M); CU: Clearfil Universal Bond Quick (Kuraray Noritake); iBU: iBond Universal (Heraeus Kulzer), then subdivided according to phosphoric acid etching time into three subgroups (SG): SG1: 0s; SG2: 15s; SG3: 30s.
Sci Adv
January 2025
Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P. R. China.
It is urgent for patients with chronic kidney disease (CKD) to develop a robust and facile therapy for effective control of serum phosphate and reasonable regulation of gut microbiota, which are aiming to prevent cardiovascular calcification and reduce cardiovascular complications. Here, bioinspired by intestinal microstructures, we developed biomimetic wrinkled prebiotic-containing microspheres with enhanced intestinal retention and absorption for reducing hyperphosphatemia and vascular calcification of CKD model rats. The resultant CSM@5 microspheres exhibited favorable phosphate binding capacity in vitro and could effectively reduce serum concentration of phosphorous in vivo.
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