Here we demonstrate the use of redox labeled double- and single-stranded oligonucleotides as recognition probes for the reagentless, single-step, electrochemical detection of anti-DNA antibodies directly in blood serum.
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http://dx.doi.org/10.1039/b922595a | DOI Listing |
Proc Natl Acad Sci U S A
January 2025
Department of Bioengineering, California Institute of Technology, Pasadena, CA 91125.
The diversity and heterogeneity of biomarkers has made the development of general methods for single-step quantification of analytes difficult. For individual biomarkers, electrochemical methods that detect a conformational change in an affinity binder upon analyte binding have shown promise. However, because the conformational change must operate within a nanometer-scale working distance, an entirely new sensor, with a unique conformational change, must be developed for each analyte.
View Article and Find Full Text PDFLangmuir
January 2025
Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS University Islamabad (CUI), Lahore Campus, Lahore 54000, Pakistan.
By integrating iron-cobalt squarate bimetallic metal-organic framework (Fe-Co-SqBMoF) based smart material (SM) with functional DNA (fDNA), we designed a target responsive fDNA@Fe-Co-SqBMoF bioelectrode that exhibits recognition induced switchable response to serve as a reagentless single step electrochemical apta-switch (REA). The construct takes advantage of fDNA ability to bind and concentrate target on the receptor interface, while Fe-Co-SqBMoF@SM multifeatures to serve as an immobilization matrix and a signal generating electrochemical switch. Fe-Co-SqBMoF was introduced to prepare a redox active pencil graphite electrode (PGE), while fDNA (aptamer) was decorated on the receptor PGE to impart specificity and selectivity.
View Article and Find Full Text PDFAnalyst
July 2024
McGill University, Department of Bioengineering, Montreal, QC, H3A 0E9, Canada.
Reagentless molecular-imprinted polymer (MIP) electrochemical biosensors can offer the next generation of biosensing platforms for the detection of biomarkers owing to their simplicity, cost-efficacy, tunability, robustness, and accuracy. In this work, a novel combination of Prussian blue (PB), coated as an embedded redox probe on a gold working electrode (GWE), and a signal-off MIP assay has been proposed in an electrochemical format for the detection of troponin I (TnI) in biofluids. TnI is a variant exclusive to heart muscles, and its elevated level in the bloodstream is indicative of acute myocardial infarction (AMI).
View Article and Find Full Text PDFACS Appl Bio Mater
June 2024
School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
C-reactive protein (CRP) is an acute-phase reactant and sensitive indicator for sepsis and other life-threatening pathologies, including systemic inflammatory response syndrome. Currently, clinical turn-around times for established CRP detection methods take between 30 min to hours or even days from centralized laboratories. Here, we report the development of an electrochemical biosensor using redox probe-tagged DNA aptamers, functionalized onto inexpensive, commercially available screen-printed electrodes.
View Article and Find Full Text PDFSensors (Basel)
November 2022
School of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada.
Soil tests for plant-available phosphorus (P) are suggested to provide offsite P analysis required to monitor P fertilizer application and reduce P losses to downstream water. However, procedural and cost limitations of current soil phosphate tests have restricted their widespread use and have made them accessible only in laboratories. This study proposes a novel paper-based reagentless electrochemical soil phosphate sensor to extract and detect soil phosphate using an inexpensive and simple approach.
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