AI Article Synopsis
- - The study assessed the effectiveness of the VP4, VP2, and VP1 gene regions in typing human enteroviruses using RT-PCR primers on 86 field isolates from different HEV types.
- - For HEV-A, all three gene regions showed 100% concordance, but only VP2 and VP1 were reliable for HEV-B and HEV-C typing, as VP4 did not perform as well for these types.
- - Further analysis of VP4 with additional sequences confirmed it aligns completely with VP1 for HEV-A, suggesting that using all three gene regions can improve virus identification, aiding in the management of diseases like hand, foot, and mouth disease.
Article Abstract
The VP4, VP2, and VP1 gene regions were evaluated for their usefulness in typing human enteroviruses. Three published RT-PCR primers sets targeting separately these three gene regions were used. Initially, from a total of 86 field isolates (36 HEV-A, 40 HEV-B, and 10 HEV-C) tested, 100% concordance in HEV-A was identified from all three gene regions (VP4, VP2, and VP1). However, for HEV-B and HEV-C viruses, only the VP2 and VP1 regions, and not VP4, showed 100% concordance in typing these viruses. To evaluate further the usefulness of VP4 in typing HEV-A enteroviruses, 55 Japanese and 203 published paired VP4 and VP1 nucleotide sequences were also examined. In each case, typing by VP4 was 100% in concordance with typing using VP1. Given these results, it is proposed that for HEV-A enteroviruses, all three gene regions (VP4, VP2, and VP1), would be useful for typing these viruses. These options would enhance the capability of laboratories in identifying these viruses and would greatly help in outbreaks of hand, foot, and mouth disease.
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| http://dx.doi.org/10.1002/jmv.21652 | DOI Listing |
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