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Nanoscale Cellular Traction Force Quantification: CRISPR-Cas12a Supercharged DNA Tension Sensors in Nanoclustered Ligand Patterns.
ACS Appl Mater Interfaces
January 2025
Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
High-throughput measurement of cellular traction forces at the nanoscale remains a significant challenge in mechanobiology, limiting our understanding of how cells interact with their microenvironment. Here, we present a novel technique for fabricating protein nanopatterns in standard multiwell microplate formats (96/384-wells), enabling the high-throughput quantification of cellular forces using DNA tension gauge tethers (TGTs) amplified by CRISPR-Cas12a. Our method employs sparse colloidal lithography to create nanopatterned surfaces with feature sizes ranging from sub 100 to 800 nm on transparent, planar, and fully PEGylated substrates.
View Article and Find Full Text PDFOffice-Based Endoscopic Urological Procedures Under Local Anesthesia: Prospective Evaluation of Feasibility, Pain, and Patient Preference.
J Endourol
January 2025
Department of Urology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Several diagnostic and therapeutic endoscopic urological procedures, such as stent placement, ureteroscopy, and bladder stone lithotripsy, can be performed in a hospital, an ambulatory surgery center, in the office with IV sedation, or in the office using only topical anesthesia. The potential benefits of performing procedures in the office setting using topical anesthesia include efficiency and cost reduction. The potential harms are failure to achieve the desired outcome and patient pain.
View Article and Find Full Text PDFCrystal Structural Editing: Novel Biaxial MgTeO Crystal as Zero-Order Waveplates.
Adv Mater
January 2025
State Key Laboratory of Crystal Materials and Institute of Crystal Materials, Shandong University, Jinan, 250100, China.
Waveplates are important optical components to control the polarization of light. Currently, they are often fabricated from uniaxial crystals, and there is no report about waveplates based on the biaxial crystals. In this work, a novel biaxial crystal MgTeO with a structure constructed by 0D TeO groups is designed and grown as waveplate materials for the first time.
View Article and Find Full Text PDFStretchable Blue Phase Liquid Crystal Lasers with Optical Stability Based on Small-Strain Nonlinear 3D Asymmetric Deformation.
Adv Mater
January 2025
CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Blue phase liquid crystal (BPLC) lasers exhibit exceptional optical quality and tunability to external stimuli, holding significant promise for innovative developments in the field of flexible optoelectronics. However, there remain challenges for BPLC elastomer (BPLCE) lasers in maintaining good optical stability during stretching and varying temperature conditions. In this work, a stretchable laser is developed based on a well-designed BPLCE with a combination of partially and fully crosslinked networks, which can output a single-peak laser under small deformation (44.
View Article and Find Full Text PDFThe influence of tooth shade on near-infrared light transmission through human dentine and enamel: an in-vitro study.
Lasers Med Sci
January 2025
School of Medicine and Dentistry, Griffith University, Gold Coast, QLD, Australia.
This in-vitro study assessed the influence of the shade of human teeth on the transmission of near-infrared light. A total of 40 teeth were used. After cleaning the root surface and removing cementum, the teeth were sectioned into slices 3 mm thick, with each comprising a portion of the crown (enamel-dentine (ED)) and of the root (dentine only).
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