AI Article Synopsis
- The study focused on identifying and characterizing the Babesia spp. responsible for canine babesiosis in Croatia, particularly in 28 symptomatic dogs.
- The researchers found that all blood smears contained Babesia canis, and PCR confirmed the presence of the subspecies Babesia canis canis in 27 out of 28 samples.
- Genetic analysis revealed high similarities among the isolates, indicating a potential new variant of B. canis canis in the infected dogs.
Article Abstract
The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with B. canis canis . The results of this study confirm the presence of B. canis canis in infected dogs in Croatia and demonstrate a slightly new genetic variant of Babesia subspecies.
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| http://dx.doi.org/10.1556/AVet.58.2010.1.4 | DOI Listing |
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