Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum.

J Biosci Bioeng

Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo 060-8628, Japan.

Published: March 2010

The effects of heat treatment and concentration of fish serum (FS) on cell growth in a suspension culture of recombinant Chinese hamster ovary (CHO) 1-15(500) (ATCC CRL-9606) cells were investigated. An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density.

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http://dx.doi.org/10.1016/j.jbiosc.2009.08.481DOI Listing

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