Ecdysteroid signaling is an important regulator of arthropod development and reproduction. However, the role of ecdysteroid signaling in another Ecdysozoan animal, the nematode, remains unclear. We report here the identification, cloning, and temporal expression of genes encoding putative homologs of the two nuclear receptor components of the ecdysone receptor, RXR/Usp (NR2B) and EcR (NR1H), in the nematode Pristionchus pacificus. The P. pacificus genes Ppa-pnhr-1 and Ppa-pnhr-2 encode nuclear receptors with strong sequence similarity to RXR/Usp and EcR, respectively. Maximum likelihood analysis incorporating both DNA-binding and ligand-binding domains places the two proteins in the NR2B and NR1H groups with strong bootstrap support. RT-PCR analysis reveals that both Ppa-pnhr-1 and Ppa-pnhr-2 are expressed during larval development and that Ppa-pnhr-1 expression oscillates with the molting cycle. The identification of a putative ecdysone receptor in a nematode amenable to genetic analysis provides a powerful system to investigate the function and evolution of ecdysone receptor signaling in the Nematoda.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ygcen.2010.02.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The ecdysone-induced bZIP transcription factor MafB establishes a positive feedback loop to enhance vitellogenesis and reproduction in the mosquito.
Proc Natl Acad Sci U S A
January 2025
Department of Entomology, University of California, Riverside, CA 92521.
Female mosquitoes require a vertebrate blood meal to activate reproduction, transmitting numerous devastating human diseases. Vitellogenesis is a central event of female reproduction that involves the massive production of vitellogenin (Vg) in the fat body and the maturation of ovaries. This process is controlled by the steroid hormone 20-hydroxyecdysone (20E); however, its molecular regulatory basis remains not completely understood.
View Article and Find Full Text PDFImplications of cyantraniliprole sublethal doses on the population dynamics and gene expression of Aphis gossypii Glover (Hemiptera: Aphididae).
Comp Biochem Physiol C Toxicol Pharmacol
December 2024
Zhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China; National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China. Electronic address:
Cyantraniliprole (CYA), widely recognized as a highly effective solution, is widely used in pest management. It has been broadly utilized to manage diverse pests, among which Aphis gossypii Glover (Hemiptera: Aphididae) is a prominent agricultural pest that leads to significant crop damage worldwide. Studies suggest that the sublethal effect of insecticides might contribute to the resurgence of A.
View Article and Find Full Text PDF20-Hydroxyecdysone Mediates Reproductive Diapause in via Ecdysone Receptor and Nuclear Hormone Receptor .
Int J Mol Sci
December 2024
Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China.
20-hydroxyecdysone (20E) signaling plays an important role in regulating insect growth, development, and reproduction. However, the effect of 20E on reproductive diapause and its regulatory mechanisms have not been fully understood. is a new pest in the Inner Mongolia grasslands, and it aestivates in an obligatory reproductive diapause form.
View Article and Find Full Text PDFAstrocyte-dependent local neurite pruning in Beat-Va neurons.
J Cell Biol
January 2025
Vollum Institute, Oregon Health & Science University, Portland, OR, USA.
Developmental neuronal remodeling is extensive and mechanistically diverse across the nervous system. We sought to identify Drosophila pupal neurons that underwent mechanistically new types of neuronal remodeling and describe remodeling Beat-VaM and Beat-VaL neurons. We show that Beat-VaM neurons produce highly branched neurites in the CNS during larval stages that undergo extensive local pruning.
View Article and Find Full Text PDFEffects of Different Levels of Antarctic Krill Oil on the Ovarian Development of .
Animals (Basel)
November 2024
Key Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China.
Antarctic krill oil has been proven to be able to promote the ovarian development of crustaceans, but its optimal application dose and potential regulatory mechanism in are still unclear. In this study, five isonitrogenous and isolipidic diets with gradient additions of Antarctic krill oil (0%, 1.5%, 3%, 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!