AI Article Synopsis
- A study introduced an affordable platform for detecting thrombin through biomolecular interactions using surface plasmon resonance with an aptamer-based assay.
- Two types of surfaces—carboxylated cross-linked BSA (cBSA) and carboxymethylated dextran chip (CM5)—were employed to anchor the thrombin-binding aptamer.
- The cBSA-based aptasensor showed reliable selectivity, stability, and regeneration capabilities, performing comparably to the higher-end CM5 platform across both buffer and plasma samples.
Article Abstract
A novel low-cost platform to assess biomolecular interactions was investigated using surface plasmon resonance and an aptamer-based assay for thrombin detection. Gold SPR surface functionalized with a carboxylated cross-linked BSA film (cBSA) and commercially available carboxymethylated dextran chip (CM5) were used as immobilization platforms for the thrombin binding aptamer. The high end commercial instrument Biacore 3000 and a custom made FIA set-up involving TI Spreeta sensor (TSPR2K23) were used to assess different concentrations of thrombin within the range 0.1-150 nM both in buffer and in a complex matrix (plasma) using the obtained aptasensors. Based on data derived from both CM5 and cBSA platforms, the cBSA aptasensor exhibited good selectivity, stability and regeneration ability, both in buffer and in complex matrices (plasma), comparable with CM5.
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| http://dx.doi.org/10.1016/j.talanta.2009.11.023 | DOI Listing |
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