Arnolds' silver staining of Helbodella (leech) embryos.

Cold Spring Harb Protoc

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.

Published: April 2009

AI Article Synopsis

  • Arnolds' silver staining is a technique that helps to clearly see the edges of epidermal cells in embryos.
  • This method can be applied to both living and preserved Helbodella (leech) embryos.
  • For fixed embryos, a unique fixation process is necessary to maintain the integrity of the cell junctions during staining.

Article Abstract

Arnolds' silver staining is used to visualize the boundaries of epidermal cells at the surface of embryos. As described in this article, silver staining can be performed on either living or fixed Helbodella (leech) embryos. To preserve the cell junction in fixed embryos, a special fixation procedure is used.

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http://dx.doi.org/10.1101/pdb.prot5192DOI Listing

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