AI Article Synopsis
- The study aimed to identify different hemoglobin (Hb) alpha variants using a specific diagnostic method.
- A high-resolution melting (HRM) technique was employed, which successfully identified multiple Hb variants such as Hb Hekinan and Hb Constant Spring by observing differences in plotted curves.
- The research concludes that HRM analysis is an effective tool for differentiating Hb variants found in the HBA1 and HBA2 genes.
Article Abstract
Objectives: The present study was aimed at identifying hemoglobin (Hb) alpha variants.
Design And Methods: To identify Hb variants, a high-resolution melting (HRM) method was performed.
Results: The diagnostic strategy was found to be successful in identifying Hb alpha variants including HBA1:c.27G>T, (Hb Hekinan) HBA1:c.46G>C (Hb Ottawa), HBA2:c.31_33AG (Hb alpha2-globin gene codon del AG), HBA1:c.223G>C (Hb G-Taichung), HBA1:p.Phe118_Thr119insIle (Hb Phnom Penh), HBA2:c.369C>G (Hb Westmead), HBA2:c.364G>A (or HBA1) (Hb Owari), HBA2:c.377T>C (Hb Quong Sze), and HBA2:c.427T>C (Hb Constant Spring). Each Hb variant could be readily and easily identified through the difference in plotted curves. In addition, the Hb variants could be distinguished to be located at either HBA1 or HBA2 gene.
Conclusions: The HRM analysis is found to be a good tool for identifying Hb variants in alpha globin genes.
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| http://dx.doi.org/10.1016/j.clinbiochem.2010.01.011 | DOI Listing |
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