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[Genetic polymorphisms of 10 STR (AmpFlSTR SGM Plus system) loci in Gypsy population from Poland]. | LitMetric

[Genetic polymorphisms of 10 STR (AmpFlSTR SGM Plus system) loci in Gypsy population from Poland].

Ann Acad Med Stetin

Wydział Biologii Centralnego Laboratorium Kryminalistycznego Komendy Głównej Policji w Warszawie, Al. Ujazdowskie 7, 00-583 Warszawa.

Published: March 2010

Introduction: The aim of the paper was to create a database of the allele distribution at 10 STR loci (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA).

Material And Methods: The DNA were isolated from samples collected from 222 unrelated individuals of the Gypsy population from Poland. Amplification was performed using a commercial multiplex FlSTR SGM Plus kit. The amplified fragments were resolved by electrophoresis in a 5% denaturing polyacrylamid gel using tne ABI Prism 377 DNA sequencer. The expected performance of the analyzed loci for personal identification testing was estimated.

Results: All loci met the Hardy-Weinberg assumption. The combinated values of the matching probability (MP) and of the power exclusion (PE) are 3.95 x 10(-13) and 0.9999641.

Conclusions: Statistical parameters (PIC, MP, PE) showed that the examined systems are useful for forensic medicine.

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