Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Introduction: The aim of the paper was to create a database of the allele distribution at 10 STR loci (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA).
Material And Methods: The DNA were isolated from samples collected from 222 unrelated individuals of the Gypsy population from Poland. Amplification was performed using a commercial multiplex FlSTR SGM Plus kit. The amplified fragments were resolved by electrophoresis in a 5% denaturing polyacrylamid gel using tne ABI Prism 377 DNA sequencer. The expected performance of the analyzed loci for personal identification testing was estimated.
Results: All loci met the Hardy-Weinberg assumption. The combinated values of the matching probability (MP) and of the power exclusion (PE) are 3.95 x 10(-13) and 0.9999641.
Conclusions: Statistical parameters (PIC, MP, PE) showed that the examined systems are useful for forensic medicine.
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