The paper presents the results of a study of embryonic or primitive hemoglobin (HbP), by using the authors' developed immunodiffusion monovalent test system in the neonatal umbilical blood and in girls than in the boys. HbP was also detected in patients with erythema (66.7%) and acute myeloleukemia (22.22%), it was absent in those with chronic lympholeukemia. The findings suggest that the early antenatal type of hemoglobin is likely to be of diagnostic value in assessing the degree of hypoxia in the differential diagnosis of hemoblastoses.
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Invest Ophthalmol Vis Sci
January 2025
Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States.
Purpose: To investigate the presence of uridine-5'-triphosphate (UTP)-activated P2Y1-like nucleotide receptors (P2Y2R, P2Y4R, and P2Y6R) in conjunctival goblet cells (CGCs) and determine if they increase intracellular Ca2+ concentration ([Ca2+]i) and induce mucin secretion.
Methods: Adult, male rat conjunctiva was used for culture of CGCs. To investigate the expression of P2YRs, mRNA was extracted from CGCs and used for reverse transcription PCR (RT-PCR) with commercially obtained primers specific to P2Y2R, P2Y4R, and P2Y6R.
J Clin Microbiol
January 2025
Department of Internal Medicine, Section of Infectious Diseases, Erasmus University Medical Center, Rotterdam, the Netherlands.
An accurate diagnosis of invasive aspergillosis (IA) in patients with underlying hematological malignancies relies heavily on galactomannan detection. In this study, we compared the VirCLIA chemiluminescence immunoassay (CLIA) with the frequently used Platelia enzyme-linked immunosorbent assay (ELISA) on serum from hematology patients with suspected IA. Patients were categorized according to EORTC/MSGERC 2020 definitions into proven/probable IA and possible/no IA.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, PR China; Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, 200032, PR China. Electronic address:
Background: Entamoeba histolytica is a parasite that could cause severe amebiasis, an extremely contagious parasitic disease with critical clinical symptoms. Timely diagnosis and treatment of E. histolytica are crucial for preventing complications and fatalities.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, Jinan, PR China; Department of Chemistry, Sungkyunkwan University, 16419, Suwon, Republic of Korea. Electronic address:
Photoelectrochemical (PEC) immunosensors are highly promising tools for monitoring biochemical molecules. Constructing high-performance heterojunctions is a general method to improve the sensitivity of PEC immunosensors. The internal electric field (IEF) formed at the heterojunction interface plays a crucial role in coordinating the separation of photogenerated carriers.
View Article and Find Full Text PDFLancet Microbe
January 2025
Emerging Pathogen Serology Group, Vaccine Development Evaluation and Preparedness Centre, UK Health Security Agency, Wiltshire, UK.
Background: In May, 2022, the first global outbreak of mpox (formerly known as monkeypox) occurred. In response, public health agencies in the UK have made smallpox vaccines available to individuals at the highest risk of infection. With mpox cases still being detected globally, novel tools are required to aid with diagnosis, serosurveillance, and the evaluation of immune responses following infection and immunisation with current and new vaccine candidates.
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