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A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454. | LitMetric
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A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454.

Niall J Lennon Robert E Lintner Scott Anderson Pablo Alvarez Andrew Barry William Brockman Riza Daza Rachel L Erlich Georgia Giannoukos Lisa Green Andrew Hollinger Cindi A Hoover David B Jaffe Frank Juhn Danielle McCarthy Danielle Perrin Karen Ponchner Taryn L Powers Kamran Rizzolo Dana Robbins

Genome Biol

Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles St, Cambridge, MA 02141, USA.

Published: August 2010

We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872875PMC
http://dx.doi.org/10.1186/gb-2010-11-2-r15DOI Listing

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