AI Article Synopsis
- - The text outlines methods for measuring the activity of matrix metalloproteinases (MMPs), which are enzymes that break down proteins like collagen, gelatin, and casein.
- - It includes detailed protocols for creating radiolabeled substrates, separating the degraded products from undegraded ones, and activating the MMPs.
- - Additionally, it discusses the pros and cons of these assays compared to immunoassays that quantify specific MMPs.
Article Abstract
The assays described allow the activity of members of the matrix metalloproteinase (MMP) family that degrade collagen, gelatin and casein substrates to be measured. The protocols described include the preparation of radiolabeled substrates, methods for the separation of degraded product from undegraded substrate, and methods for the activation of MMPs. The advantages and disadvantages of these methods are discussed in relation to immunoassays that measure the amount of individual MMPs.
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| http://dx.doi.org/10.1007/978-1-60327-299-5_15 | DOI Listing |
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