We engineered Lactobacillus paracasei to produce a dsRNA that would trigger RNAi-induced silencing of an essential gene in the nematode Caenorhabditis elegans. The dsRNA-expressing L. paracasei can be used in experiments conducted on culture plates and may also be used as an orally administrable dsRNA carrier for humans and other mammals.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbiosc.2009.08.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microbial interventions in yak colibacillosis: Lactobacillus-mediated regulation of intestinal barrier.
Front Cell Infect Microbiol
February 2024
College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, China.
Introduction: The etiology of Escherichia coli in yaks, along with its drug resistance, results in economic losses within the yak breeding industry. The utilization of lactic acid bacteria treatment has emerged as a viable alternative to antibiotics in managing colibacillosis.
Methods: To elucidate the therapeutic mechanisms of Lactobacillus against Escherichia coli-induced intestinal barrier damage in yaks, we employed yak epithelial cells as the experimental model and established a monolayer epithelial barrier using Transwell.
Tryptophan metabolite norharman secreted by cultivated Lactobacillus attenuates acute pancreatitis as an antagonist of histone deacetylases.
BMC Med
August 2023
Laboratory of Integrative Medicine, First Affiliated Hospital of Dalian Medical University, No.222 Zhongshan Road, Dalian, 116011, China.
Background: Patients with acute pancreatitis (AP) exhibit specific phenotypes of gut microbiota associated with severity. Gut microbiota and host interact primarily through metabolites; regrettably, little is known about their roles in AP biological networks. This study examines how enterobacterial metabolites modulate the innate immune system in AP aggravation.
View Article and Find Full Text PDFDifferential Expression of Mitosis and Cell Cycle Regulatory Genes during Recovery from an Acute Respiratory Virus Infection.
Pathogens
December 2021
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Acute respiratory virus infections can have profound and long-term effects on lung function that persist even after the acute responses have fully resolved. In this study, we examined gene expression by RNA sequencing in the lung tissue of wild-type BALB/c mice that were recovering from a sublethal infection with the pneumonia virus of mice (PVM), a natural rodent pathogen of the same virus family and genus as the human respiratory syncytial virus. We compared these responses to gene expression in PVM-infected mice treated with , an immunobiotic agent that limits inflammation and averts the negative clinical sequelae typically observed in response to acute infection with this pathogen.
View Article and Find Full Text PDFCarrot Juice Fermentations as Man-Made Microbial Ecosystems Dominated by Lactic Acid Bacteria.
Appl Environ Microbiol
June 2018
University of Antwerp, Research Group Environmental Ecology and Applied Microbiology (ENdEMIC), Department of Bioscience Engineering, Antwerp, Belgium
Article Synopsis
- - Spontaneous vegetable fermentations, specifically carrot juice, are becoming popular due to their flavor and potential health benefits, but their microbiological processes are not well understood, raising food safety concerns.
- - A study analyzed samples from 38 carrot juice fermentations collected via citizen science, finding that lactic acid bacteria (LAB) dominated after just a few days and produced metabolites like lactic acid and cadaverine.
- - Genetic analyses revealed a high diversity of LAB, revealing their roles in fermentation, but only certain strains maintained the fermentation dynamics, highlighting the carrot juice fermentation as a valuable system for studying LAB and their ecological interactions.
Regulation of intestinal inflammation by microbiota following allogeneic bone marrow transplantation.
J Exp Med
May 2012
Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
Article Synopsis
- Researchers found significant changes in gut microbiota associated with intestinal inflammation in both mice and human patients undergoing bone marrow transplantation (BMT) due to graft-versus-host disease (GVHD).
- The study showed that loss of gut diversity and specific microbial shifts, like the expansion of Lactobacillales, worsened GVHD, while reintroducing beneficial Lactobacillus strains offered protection.
- Increased microbial chaos shortly after BMT was identified as a potential risk factor for developing GVHD, indicating that manipulating gut flora may help reduce inflammation and enhance recovery for BMT patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!